Human LYZ / Lysozyme
(tested or 100% immunogen sequence identity)
Ion exchange chromatography
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LYZ / Lysozyme antibody was raised against human lysozyme
Human LYZ / Lysozyme. The reactivity of the antiserum is restricted to lysozyme. In immunoelectrophoresis and radial immuno- diffusion (Ouchterlony), using various antiserum concentrations against normal human milk and tears a single precipitin line is obtained which shows a reaction of identity with the precipitin line obtained with purified lysozyme. No reaction is obtained with any other plasma protein component or serum. Cross-reactivity The antiserum does not cross-react with any other component of human plasma. Inter-species cross- reactivity is a normal feature of antibodies to plasma proteins since they frequently share antigenic determinants. Cross-reactivity of this antiserum has not been tested in detail.
This product is intended for use in enzyme-immunocytochemical and immunohistochemical techniques for the detection of lysozyme at the cellular and subcellular level in appropriately treated cell and tissue substrates; as detection reagent in non-isotopic methodology and solid phase immunochemistry (e. g. ELISA, Western blotting). This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labeled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal. Working dilutions for histochemical and cytochemical use are usually between 1:100 and 1:500; in ELISA and comparable non-precipitating antibody-binding assays between 1:1000 and 1:6000. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.
Lyophilized from PBS, pH 7.2
1 ml Sterile distilled water 1 ml
Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.
For research use only.
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