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Catalog Number Size Price
LS-C313296-10 10 µg $318 
LS-C313296-100 100 µg $470 
LPXN / Leupaxin Antibody - IHC analysis of Leupaxin using anti-Leupaxin antibody. Leupaxin was detected in paraffin-embedded section of human intestinal cancer tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1µg/ml rabbit anti-Leupaxin antibody overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.
LPXN / Leupaxin Antibody - LPXN / Leupaxin antibody. IHC(P): Mouse Spleen Tissue.
LPXN / Leupaxin Antibody - LPXN / Leupaxin antibody. IHC(P): Rat Spleen Tissue.
LPXN / Leupaxin Antibody - WB of LPXN / Leupaxin antibody. Lane 1: Rat Thymus Tissue Lysate. Lane 2: JURKAT Cell Lysate.
LPXN / Leupaxin Antibody - IHC analysis of Leupaxin using anti-Leupaxin antibody. Leupaxin was detected in paraffin-embedded section of human intestinal cancer tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1µg/ml rabbit anti-Leupaxin antibody overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.
LPXN / Leupaxin Antibody - LPXN / Leupaxin antibody. IHC(P): Mouse Spleen Tissue.
LPXN / Leupaxin Antibody - LPXN / Leupaxin antibody. IHC(P): Rat Spleen Tissue.
LPXN / Leupaxin Antibody - WB of LPXN / Leupaxin antibody. Lane 1: Rat Thymus Tissue Lysate. Lane 2: JURKAT Cell Lysate.
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Polyclonal Rabbit anti‑Human LPXN / Leupaxin Antibody (aa115‑129, IHC, WB) LS‑C313296

Polyclonal Rabbit anti‑Human LPXN / Leupaxin Antibody (aa115‑129, IHC, WB) LS‑C313296

Note: This antibody replaces LS-C388783
Antibody:
LPXN / Leupaxin Rabbit anti-Human Polyclonal (aa115-129) Antibody
Application:
IHC, IHC-P, WB
Reactivity:
Human, Monkey, Mouse, Rat
Format:
Unconjugated, Unmodified
Price
Catalog Number
$318
LS-C313296-10
Toll Free North America
206-374-1102
For Research Use Only

Overview

Antibody:
LPXN / Leupaxin Rabbit anti-Human Polyclonal (aa115-129) Antibody
Application:
IHC, IHC-P, WB
Reactivity:
Human, Monkey, Mouse, Rat
Format:
Unconjugated, Unmodified

Specifications

Description
Leupaxin antibody LS-C313296 is an unconjugated rabbit polyclonal antibody to Leupaxin (LPXN) (aa115-129) from human. It is reactive with human, mouse, rat and other species. Validated for IHC and WB. Cited in 1 publication.
Target
Human LPXN / Leupaxin
Synonyms
LPXN | LDLP | Leupaxin | LDPL
Host
Rabbit
Reactivity
Human, Monkey, Mouse, Rat (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Immunogen affinity purified
Modifications
Unmodified
Immunogen
A synthetic peptide corresponding to a sequence at the N-Terminus of human Leupaxin(115-129 aa KKHLPDKQDHKASLD), different from the related rat sequence by two amino acids, and from the related mouse sequence by three amino acids.
Epitope
aa115-129
Specificity
Macrophages, monocytes and osteoclasts (at protein level). Strongly expressed in cells and tissues of hematopoietic origin. Highest expression in lymphoid tissues such as spleen, lymph node, thymus and appendix and in the vascular smooth muscle. Lower levels in bone marrow and fetal liver. Also expressed in peripheral blood lymphocytes and a number of hematopoietic cell lines. Very low levels found in epithelial cell lines. Expressed in prostate cancer (PCa) cells and its expression intensity is directly linked to PCa progression. .
Applications
  • IHC
  • IHC - Paraffin
  • Western blot
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Presentation
Lyophilized from 0.2mg Na2HPO4, 5mg BSA, 0.9mg NaCl, 0.05mg Thimerosal, 0.05mg sodium azide.
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500µg/ml.
Storage
At -20°C for 1 year. After reconstitution, at 4°C for 1 month. It can also be aliquotted and stored frozen at -20°C for a longer time. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About LPXN / Leupaxin
O60711 NM_004811 NP_004802.1

LSBio Ratings

LPXN / Leupaxin Antibody (aa115-129) for IHC, WB/Western LS-C313296 has an LSBio Rating of
Publications (4)

Publications (1)

Protein tyrosine kinase 2b inhibition reverts niche-associated resistance to tyrosine kinase inhibitors in AML. Catana Allert, Alexander Waclawiczek , Sarah Miriam Naomi Zimmermann , Stefanie Göllner, Daniel Heid, Maike Janssen, Simon Renders, Christian Rohde, Marcus Bauer, Margarita Bruckmann, Rafael Zinz, Cornelius Pauli, Birgit Besenbeck, Claudia Wickenhauser, Andreas Trumpp, Jeroen Krijgsveld, Carsten Müller-Tidow, Maximilian Felix Blank. Leukemia. 2022 October;36:2418-2429.

Customer Reviews (0)


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Requested From: United States
Date Requested: 4/26/2024