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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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LOX / Lysyl Oxidase Antibody (clone 6B7) LS‑C114936
Lysyl Oxidase antibody LS-C114936 is an unconjugated mouse monoclonal antibody to human Lysyl Oxidase (LOX). Validated for Flow, IF, IHC and WB.
Catalog
Size
Price
LS-C114936-100
100 µl (0.6 mg/ml)
Unavailable

Popular LOX / Lysyl Oxidase Products

Human placental villi, 40X. Staining of placental trophoblasts using antibody at 10 ug/ml.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Species: Human, Mouse, Rat, Bovine, Pig
Applications: IHC, IHC - Paraffin, Western blot
LOX propeptide Antibody - IHC of LOPP in mouse intestine.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Species: Mouse, Human, Rat, Pig, Primate
Applications: IHC, IHC - Paraffin
Western blot of LOX in A) human skin and B) mouse ear skin lysate using LS-C148572 at 1:500. Goat anti-rabbit Ig HRP secondary antibody, and PicoTect ECL substrate solution, were used for this test.
Species: Human, Monkey, Mouse, Bat, Rabbit
Applications: IHC, IHC - Frozen, Western blot, Immunoprecipitation
Anti-Lysyl Oxidase / LOX antibody IHC of human skin. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B8037 dilution 1:100.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Species: Human, Rat
Applications: IHC, IHC - Paraffin, ICC, Immunofluorescence, Western blot, Flow Cytometry
IHC of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-LOX mouse monoclonal antibody.
Species: Human
Applications: IHC, IHC - Paraffin, Immunofluorescence, Western blot, Flow Cytometry

Product Description

Lysyl Oxidase antibody LS-C114936 is an unconjugated mouse monoclonal antibody to human Lysyl Oxidase (LOX). Validated for Flow, IF, IHC and WB.
About LOX / Lysyl Oxidase
Lysyl Oxidase / LOX is an extracellular copper enzyme that initiates the crosslinking of collagens and elastin. The enzyme catalyzes oxidative deamination of the epsilon-amino group in certain lysine and hydroxylysine residues of collagens and lysine residues of elastin. In addition to crosslinking extracellular matrix proteins, the encoded protein may have a role in tumor suppression. Defects in this gene are a cause of autosomal recessive cutis laxa type I (CL type I). P28300 NM_002317 NP_002308.2

LOX Antibody, Lysyl oxidase Antibody, Protein-lysine 6-oxidase Antibody


Specifications

Target
Human LOX / Lysyl Oxidase
Host
Mouse
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
IgG1 Monoclonal [6B7]
Conjugations
Unconjugated
Purification
Protein A/G purified
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin (1:50)
  • Immunofluorescence (1:100)
  • Western blot (1:1000)
  • Flow Cytometry (1:100)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
Human recombinant protein fragment corresponding to amino acids 22-168 of human LOX (NP_002308) produced in HEK293T cell.
Specificity
Human Lysyl Oxidase / LOX
Usage
Concentration: 0.5-1.0 mg/ml (Lot dependent)
Presentation
PBS, pH 7.3, 1% BSA, 50% glycerol, 0.02% sodium azide
Storage
Store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.

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Images


Immunohistochemistry

IHC of paraffin-embedded Ovary tissue using anti-LOX mouse monoclonal antibody. (Dilution 1:50).
IHC of paraffin-embedded Ovary tissue using anti-LOX mouse monoclonal antibody. (Dilution 1:50).

Immunohistochemistry

IHC of paraffin-embedded liver tissue using anti-LOX mouse monoclonal antibody. (Dilution 1:50).
IHC of paraffin-embedded liver tissue using anti-LOX mouse monoclonal antibody. (Dilution 1:50).

Immunofluorescence

Anti-LOX mouse monoclonal antibody  immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY LOX.
Anti-LOX mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY LOX.

Western blot

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY LOX (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-LOX.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY LOX (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-LOX.

Flow Cytometry

Flow cytometry of Jurkat cells, using anti-LOX antibody, (Red) compared to a nonspecific negative control antibody (Blue).
Flow cytometry of Jurkat cells, using anti-LOX antibody, (Red) compared to a nonspecific negative control antibody (Blue).

Flow Cytometry

Flow cytometry of HeLa cells, using anti-LOX antibody, (Red) compared to a nonspecific negative control antibody (Blue).
Flow cytometry of HeLa cells, using anti-LOX antibody, (Red) compared to a nonspecific negative control antibody (Blue).

Flow Cytometry

HEK293T cells transfected with either pCMV6-ENTRY LOX (Red) or empty vector control plasmid (Blue) were immunostained with anti-LOX mouse monoclonal, and then analyzed by flow cytometry.
HEK293T cells transfected with either pCMV6-ENTRY LOX (Red) or empty vector control plasmid (Blue) were immunostained with anti-LOX mouse monoclonal, and then analyzed by flow cytometry.

Immunohistochemistry

IHC of paraffin-embedded Ovary tissue using anti-LOX mouse monoclonal antibody. (Dilution 1:50).
IHC of paraffin-embedded Ovary tissue using anti-LOX mouse monoclonal antibody. (Dilution 1:50).

Immunohistochemistry

IHC of paraffin-embedded liver tissue using anti-LOX mouse monoclonal antibody. (Dilution 1:50).
IHC of paraffin-embedded liver tissue using anti-LOX mouse monoclonal antibody. (Dilution 1:50).

Immunofluorescence

Anti-LOX mouse monoclonal antibody  immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY LOX.
Anti-LOX mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY LOX.

Western blot

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY LOX (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-LOX.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY LOX (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-LOX.

Flow Cytometry

Flow cytometry of Jurkat cells, using anti-LOX antibody, (Red) compared to a nonspecific negative control antibody (Blue).
Flow cytometry of Jurkat cells, using anti-LOX antibody, (Red) compared to a nonspecific negative control antibody (Blue).

Flow Cytometry

Flow cytometry of HeLa cells, using anti-LOX antibody, (Red) compared to a nonspecific negative control antibody (Blue).
Flow cytometry of HeLa cells, using anti-LOX antibody, (Red) compared to a nonspecific negative control antibody (Blue).

Flow Cytometry

HEK293T cells transfected with either pCMV6-ENTRY LOX (Red) or empty vector control plasmid (Blue) were immunostained with anti-LOX mouse monoclonal, and then analyzed by flow cytometry.
HEK293T cells transfected with either pCMV6-ENTRY LOX (Red) or empty vector control plasmid (Blue) were immunostained with anti-LOX mouse monoclonal, and then analyzed by flow cytometry.

Immunohistochemistry

IHC of paraffin-embedded Ovary tissue using anti-LOX mouse monoclonal antibody. (Dilution 1:50).
IHC of paraffin-embedded Ovary tissue using anti-LOX mouse monoclonal antibody. (Dilution 1:50).

Immunohistochemistry

IHC of paraffin-embedded liver tissue using anti-LOX mouse monoclonal antibody. (Dilution 1:50).
IHC of paraffin-embedded liver tissue using anti-LOX mouse monoclonal antibody. (Dilution 1:50).

Immunofluorescence

Anti-LOX mouse monoclonal antibody  immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY LOX.
Anti-LOX mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY LOX.

Western blot

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY LOX (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-LOX.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY LOX (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-LOX.

Flow Cytometry

Flow cytometry of Jurkat cells, using anti-LOX antibody, (Red) compared to a nonspecific negative control antibody (Blue).
Flow cytometry of Jurkat cells, using anti-LOX antibody, (Red) compared to a nonspecific negative control antibody (Blue).

Flow Cytometry

Flow cytometry of HeLa cells, using anti-LOX antibody, (Red) compared to a nonspecific negative control antibody (Blue).
Flow cytometry of HeLa cells, using anti-LOX antibody, (Red) compared to a nonspecific negative control antibody (Blue).

Flow Cytometry

HEK293T cells transfected with either pCMV6-ENTRY LOX (Red) or empty vector control plasmid (Blue) were immunostained with anti-LOX mouse monoclonal, and then analyzed by flow cytometry.
HEK293T cells transfected with either pCMV6-ENTRY LOX (Red) or empty vector control plasmid (Blue) were immunostained with anti-LOX mouse monoclonal, and then analyzed by flow cytometry.

Immunohistochemistry

IHC of paraffin-embedded Ovary tissue using anti-LOX mouse monoclonal antibody. (Dilution 1:50).
IHC of paraffin-embedded Ovary tissue using anti-LOX mouse monoclonal antibody. (Dilution 1:50).

Immunohistochemistry

IHC of paraffin-embedded liver tissue using anti-LOX mouse monoclonal antibody. (Dilution 1:50).
IHC of paraffin-embedded liver tissue using anti-LOX mouse monoclonal antibody. (Dilution 1:50).

Immunofluorescence

Anti-LOX mouse monoclonal antibody  immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY LOX.
Anti-LOX mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY LOX.

Western blot

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY LOX (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-LOX.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY LOX (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-LOX.

Flow Cytometry

Flow cytometry of Jurkat cells, using anti-LOX antibody, (Red) compared to a nonspecific negative control antibody (Blue).
Flow cytometry of Jurkat cells, using anti-LOX antibody, (Red) compared to a nonspecific negative control antibody (Blue).

Flow Cytometry

Flow cytometry of HeLa cells, using anti-LOX antibody, (Red) compared to a nonspecific negative control antibody (Blue).
Flow cytometry of HeLa cells, using anti-LOX antibody, (Red) compared to a nonspecific negative control antibody (Blue).

Flow Cytometry

HEK293T cells transfected with either pCMV6-ENTRY LOX (Red) or empty vector control plasmid (Blue) were immunostained with anti-LOX mouse monoclonal, and then analyzed by flow cytometry.
HEK293T cells transfected with either pCMV6-ENTRY LOX (Red) or empty vector control plasmid (Blue) were immunostained with anti-LOX mouse monoclonal, and then analyzed by flow cytometry.


Requested From: 
Date Requested: 6/24/2018

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