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Anti-LIMK1 / LIMK Antibody (clone 3A3) LS-C173129

Ordering

Wt. Vol. Conc. Price
- 100 µl 0.73 mg/ml $325
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)
206-374-1102
866-206-6909
Orders@LSBio.com
 

Most Popular LIMK1 / LIMK Antibodies

Anti-LIMK1 / LIMK Antibody (clone 1A8) IHC-plus™ LS-B5430
Mouse Monoclonal [clone 1A8] (IgG2a,k) to Human LIMK1 / LIMK
Human, Mouse, Rat
IHC - Paraffin, ELISA
Unconjugated
Immunohistochemistry Image
Anti-LIMK1 / LIMK Antibody (clone 2E9) IHC-plus™ LS-B5431
Mouse Monoclonal [clone 2E9] (IgG2a,k) to Human LIMK1 / LIMK
Human
IHC - Paraffin, Western blot, ELISA
Unconjugated
Immunohistochemistry Image
Anti-LIMK1 / LIMK Antibody (clone 6B4) LS-C173012
Mouse Monoclonal [clone 6B4] (IgG2a) to Human LIMK1 / LIMK
Human, Monkey, Mouse, Rat, Dog
IHC - Paraffin, Immunofluorescence, Western blot, Flow Cytometry
Unconjugated
Immunohistochemistry Image

100% Guaranteed 100% Guaranteed
Mouse Monoclonal [clone 3A3] (IgG2a) to Human LIMK1 / LIMK
Human
Immunofluorescence, Western blot, Flow Cytometry
Unconjugated

Details

Human LIMK1 / LIMK
Mouse
Human (tested or 100% immunogen sequence identity)
IgG2a Monoclonal [3A3]
Unconjugated
Protein A/G purified
Unmodified

Applications

  • Immunofluorescence (1:100)
  • Western blot (1:2000)
  • Flow Cytometry (1:100)

Specificity and Use

LIMK1 / LIMK antibody was raised against full length human recombinant protein of human LIMK1(NP_002305) produced in HEK293T cell.
Human LIMK1 / LIMK

Packaging

PBS, pH 7.3, 1% BSA, 50% glycerol, 0.02% sodium azide
Store at -20°C. Minimize freezing and thawing.
For research use only.

About LIMK1 / LIMK

P53667 NM_002314 NP_002305.1

LIMK1 Antibody, LIM domain kinase 1 Antibody, Lim kinase 1 Antibody, LIMK-1 Antibody, LIM kinase Antibody, LIMK Antibody

Serine/threonine-protein kinase that plays an essential role in the regulation of actin filament dynamics. Acts downstream of several Rho family GTPase signal transduction pathways. Activated by upstream kinases including ROCK1, PAK1 and PAK4, which phosphorylate LIMK1 on a threonine residue located in its activation loop.

Immunofluorescence

Immunofluorescence
Anti-LIMK1 mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY LIMK1.

Western blot

Western blot
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY LIMK1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-LIMK1.

Flow Cytometry

Flow Cytometry
HEK293T cells transfected with either overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-LIMK1 antibody, and then analyzed by flow cytometry.
 

Requested From: United States
Date Requested: 12/5/2016

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