Human LILRB5 / LIR8

Goat

Human (tested or 100% immunogen sequence identity)

IgG Polyclonal

Unconjugated

Affinity purified

Unmodified

NS0-derived rhLIR-8 (aa18-456) isoform 1.

aa18-456

Selected for its ability to recognize human LIR-8 in the applications listed. In direct ELISAs and Western Blots, this antibody shows less than 5% cross-reactivity with rhILT-1, -2, -3, -4, -5, and rhLIR-6.

• Western blot
• Flow Cytometry
• ELISA

Western Blot: This antibody can be used at 0.1-0.2 ug/ml with the appropriate secondary reagents to detect human LIR-8. The detection limit for rhLIR-8 is approximately 5 ng/lane under non-reducing and reducing conditions. Flow Cytometry: This antibody has been tested on whole blood monocytes for use in flow cytometry. Dilute this antibody to 50 ug/ml and add 10 ul of the diluted solution to 1-2.5 x 10^5 cells in a total reaction volume not exceeding 200 L. The binding of unlabeled antibodies may be visualized by adding a secondary developing reagent such as anti-goat IgG conjugated to a fluorochrome. Direct ELISA: This antibody can be used at 0.5-1.0 ug/ml with the appropriate secondary reagents to detect human LIR-8. The detection limit for rhLIR-8 is approximately 0.3 ng/well.

Lyophilized

Reconstitute in deionized water

For research use only. Intended for use by laboratory professionals.

This antibody carries the LSBio 100% Guarantee.

Uniprot: O75023 NCBI: NM_006840 NP_006831.1