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Antibodies > LGALS3 / Galectin 3 > LS-C343950
 
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Catalog Number Size Price
LS-C343950-10 10 µg $318 
LS-C343950-100 100 µg (0.5 mg/ml) $470 
LGALS3 / Galectin 3 Antibody - IHC analysis of Galectin 3 using anti-Galectin 3 antibody. Galectin 3 was detected in paraffin-embedded section of rat liver tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1µg/ml rabbit anti-Galectin 3 Antibody overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.
LGALS3 / Galectin 3 Antibody - IHC analysis of Galectin 3 using anti-Galectin 3 antibody. Galectin 3 was detected in paraffin-embedded section of mouse intestine tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1µg/ml rabbit anti-Galectin 3 Antibody overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.
LGALS3 / Galectin 3 Antibody - Galectin 3 antibody IHC-paraffin: Human Lung Cancer Tissue.
LGALS3 / Galectin 3 Antibody - Galectin 3 antibody IHC-paraffin: Human Placenta Tissue.
LGALS3 / Galectin 3 Antibody - IF analysis of Galectin-3 using anti-Galectin-3 antibody Galectin-3 was detected in immunocytochemical section of U20S cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent for 15 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2µg/mL rabbit anti-Galectin-3 Antibody overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
LGALS3 / Galectin 3 Antibody - Western blot analysis of Galectin 3 using anti-Galectin 3 antibody. Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat ovarian tissue lysates, Lane 2: mouse RAW264. 7 whole cell lysates, After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Galectin 3 antigen affinity purified polyclonal antibody at 0.5 µg/mL overnight at 4°C, then washed with TBS-0.1% Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for Galectin 3 at approximately 29KD. The expected band size for Galectin 3 is at 26KD.
LGALS3 / Galectin 3 Antibody - Western blot analysis of Galectin-3 using anti-Galectin-3 antibody. Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: Hela Whole Cell Lysate Lane 2: SGC Whole Cell Lysate Lane 3: MCF-7 Whole Cell Lysate Lane 4: SW620 Whole Cell Lysate Lane 5: A375 Whole Cell Lysate Lane 6: Smmc Whole Cell Lysate After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Galectin-3 antigen affinity purified polyclonal antibody at 0.5 µg/mL overnight at 4°C, then washed with TBS-0.1% Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for Galectin-3 at approximately 26KD. The expected band size for Galectin-3 is at 26KD.
LGALS3 / Galectin 3 Antibody - Flow Cytometry analysis of U20S cells using anti-Galectin-3 antibody. Overlay histogram showing U20S cells stained with anti-Galectin-3 antibody (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Galectin-3 Antibody (1µg/10E6 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (5-10µg/10E6 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1µg/10E6 cells) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
LGALS3 / Galectin 3 Antibody - IHC analysis of Galectin 3 using anti-Galectin 3 antibody. Galectin 3 was detected in paraffin-embedded section of rat liver tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1µg/ml rabbit anti-Galectin 3 Antibody overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.
LGALS3 / Galectin 3 Antibody - IHC analysis of Galectin 3 using anti-Galectin 3 antibody. Galectin 3 was detected in paraffin-embedded section of mouse intestine tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1µg/ml rabbit anti-Galectin 3 Antibody overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.
LGALS3 / Galectin 3 Antibody - Galectin 3 antibody IHC-paraffin: Human Lung Cancer Tissue.
LGALS3 / Galectin 3 Antibody - Galectin 3 antibody IHC-paraffin: Human Placenta Tissue.
LGALS3 / Galectin 3 Antibody - IF analysis of Galectin-3 using anti-Galectin-3 antibody Galectin-3 was detected in immunocytochemical section of U20S cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent for 15 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2µg/mL rabbit anti-Galectin-3 Antibody overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
LGALS3 / Galectin 3 Antibody - Western blot analysis of Galectin 3 using anti-Galectin 3 antibody. Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat ovarian tissue lysates, Lane 2: mouse RAW264. 7 whole cell lysates, After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Galectin 3 antigen affinity purified polyclonal antibody at 0.5 µg/mL overnight at 4°C, then washed with TBS-0.1% Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for Galectin 3 at approximately 29KD. The expected band size for Galectin 3 is at 26KD.
LGALS3 / Galectin 3 Antibody - Western blot analysis of Galectin-3 using anti-Galectin-3 antibody. Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: Hela Whole Cell Lysate Lane 2: SGC Whole Cell Lysate Lane 3: MCF-7 Whole Cell Lysate Lane 4: SW620 Whole Cell Lysate Lane 5: A375 Whole Cell Lysate Lane 6: Smmc Whole Cell Lysate After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Galectin-3 antigen affinity purified polyclonal antibody at 0.5 µg/mL overnight at 4°C, then washed with TBS-0.1% Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for Galectin-3 at approximately 26KD. The expected band size for Galectin-3 is at 26KD.
LGALS3 / Galectin 3 Antibody - Flow Cytometry analysis of U20S cells using anti-Galectin-3 antibody. Overlay histogram showing U20S cells stained with anti-Galectin-3 antibody (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Galectin-3 Antibody (1µg/10E6 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (5-10µg/10E6 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1µg/10E6 cells) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
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Polyclonal Rabbit anti‑Human LGALS3 / Galectin 3 Antibody (aa139‑250, IHC, WB) LS‑C343950

Polyclonal Rabbit anti‑Human LGALS3 / Galectin 3 Antibody (aa139‑250, IHC, WB) LS‑C343950

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Note: This antibody replaces LS-C388486
Antibody:
LGALS3 / Galectin 3 Rabbit anti-Human Polyclonal (aa139-250) Antibody
Application:
IHC, IHC-P, WB
Reactivity:
Human
Format:
Unconjugated, Unmodified
Price
Catalog Number
$318
LS-C343950-10
Toll Free North America
206-374-1102
For Research Use Only
Specifications Validation Publications Reviews Featured Products Request SDS/MSDS

Overview

Antibody:
LGALS3 / Galectin 3 Rabbit anti-Human Polyclonal (aa139-250) Antibody
Application:
IHC, IHC-P, WB
Reactivity:
Human
Format:
Unconjugated, Unmodified

Specifications

Description
Galectin 3 antibody LS-C343950 is an unconjugated rabbit polyclonal antibody to human Galectin 3 (LGALS3) (aa139-250). Validated for IHC and WB.
Target
Human LGALS3 / Galectin 3
Synonyms
LGALS3 | 35 kd lectin | 35 kDa lectin | CBP35 | GAL3 | Galactoside-binding protein | GALIG | IgE-binding protein | Laminin-binding protein | Gal-3 | GALBP | MAC-2 | Mac-2 antigen | L31 | MAC2 | CBP 35 | Galactose-specific lectin 3 | Galectin-3 | L-31 | Lectin L-29
Host
Rabbit
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Immunogen affinity purified
Modifications
Unmodified
Immunogen
E.coli-derived human Galectin 3 recombinant protein (Position: K139-I250). Human Galectin 3 shares 88% and 84% amino acid (aa) sequences identity with mouse and rat Galectin 3, respectively.
Epitope
aa139-250
Specificity
A major expression is found in the colonic epithelium. It is also abundant in the activated macrophages. Expressed in fetal membranes. .
Applications
  • IHC
  • IHC - Paraffin
  • Western blot
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Presentation
Lyophilized from 0.2mg Na2HPO4, 0.9mg NaCl, 0.05mg sodium azide, 4mg Trehalose
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500µg/ml.
Storage
At -20°C for 1 year. After reconstitution, at 4°C for 1 month. It can also be aliquotted and stored frozen at -20°C for a longer time. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About LGALS3 / Galectin 3
P17931 NM_002306 NP_002297.2

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Featured Products

LGALS3 / Galectin 3 Antibody - Anti-Galectin-3 antibody IHC of human small intestine. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested.
PathPlus™ LGALS3 / Galectin 3 Antibody (aa38‑87) LS‑B4682
Species: Human, Mouse
Applications: IHC, IHC - Paraffin, Western blot
LGALS3 / Galectin 3 Antibody - Human Small Intestine: Formalin-Fixed, Paraffin-Embedded (FFPE)
LGALS3 / Galectin 3 Antibody (aa1‑58, clone A3A12) LS‑C7048
Species: Human, Mouse, Rat
Applications: IHC, IHC - Paraffin, Western blot, Flow Cytometry, ELISA
LGALS3 / Galectin 3 Antibody - Anti-LGALS3 / Galectin 3 antibody IHC staining of human small intestine. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
IHC‑plus™ LGALS3 / Galectin 3 Antibody (clone 1G8) LS‑B9410
Species: Human
Applications: IHC, IHC - Paraffin, Immunofluorescence, Western blot
LGALS3 / Galectin 3 Antibody - Anti-Galectin-3 antibody IHC of human spleen, macrophages. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:100.
PathPlus™ LGALS3 / Galectin 3 Antibody (aa141‑190) LS‑B5661
Species: Human, Mouse, Rat
Applications: IHC, IHC - Paraffin, Immunofluorescence, Western blot, Peptide Enzyme-Linked Immunosorbent Assay
LGALS3 / Galectin 3 Antibody - Anti-LGALS3 / Galectin 3 antibody IHC staining of human colon. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
IHC‑plus™ LGALS3 / Galectin 3 Antibody (aa155‑168) LS‑B9435
Species: Mouse, Human
Applications: IHC, IHC - Paraffin, Western blot, Peptide Enzyme-Linked Immunosorbent Assay
LGALS3 / Galectin 3 Antibody - Immunohistochemistry of paraffin-embedded rat brain using LGALS3 Antibodyat dilution of 1:200 (40x lens).
LGALS3 / Galectin 3 Antibody LS‑C331481
Species: Human, Mouse, Rat
Applications: IHC, Immunofluorescence, Western blot
See all LGALS3 / Galectin 3 Antibodies

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com
Requested From: United States
Date Requested: 4/25/2024
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