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Anti-LCN2 / Lipocalin 2 / NGAL Antibody (aa60-72) IHC-plus™ LS-B2496

Note: This antibody replaces LS-C55067, LS-C71708, LS-C125878

Ordering

Wt. Vol. Conc. Price
50 µg - 0.5 mg/ml $395
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)
206-374-1102
866-206-6909
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Most Popular LCN2 / Lipocalin 2 / NGAL Antibodies

Anti-LCN2 / Lipocalin 2 / NGAL Antibody (clone 5G5) LS-C16216
Mouse Monoclonal [clone 5G5] (IgG1,k) to Human LCN2 / Lipocalin 2 / NGAL
Human
IHC - 4% paraformaldehyde, Western blot, ELISA
Unconjugated
Anti-LCN2 / Lipocalin 2 / NGAL Antibody LS-C16217
Mouse Monoclonal (IgG1,k) to Human LCN2 / Lipocalin 2 / NGAL
Human
IHC, Western blot, ELISA, Sandwich ELISA
Unconjugated
Anti-LCN2 / Lipocalin 2 / NGAL Antibody (clone GF1) LS-C189942
Mouse Monoclonal [clone GF1] (IgG1,k) to Human LCN2 / Lipocalin 2 / NGAL
Human
Western blot, ELISA
Unconjugated

100% Guaranteed 100% Guaranteed Publications Publications (3)
Goat Polyclonal to Human LCN2 / Lipocalin 2 / NGAL
Human
IHC - Paraffin, Western blot, ELISA
Unconjugated

Details

Human LCN2 / Lipocalin 2 / NGAL
Goat
Human (tested or 100% immunogen sequence identity)
Polyclonal
Unconjugated
Immunoaffinity purified
Unmodified

Applications

  • IHC - Paraffin (2.5 µg/ml)
  • Western blot (1:16000) & (0.01 - 0.03 µg/ml)
  • ELISA (1:16000)

Specificity and Use

LCN2 / Lipocalin 2 / NGAL antibody was raised against synthetic peptide C-AILREDKDPQKMY from an internal region of human LCN2 (NP_005555.2). Percent identity by BLAST analysis: Human, Gorilla (100%).
LS-E25880 - Lyophilized - 100 µg - $145.00
aa60-72
Human LCN2.
Immunohistochemistry: LS-B2496 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B2496 was determined to be 2.5 ug/ml.

Packaging

Tris-buffered saline, pH 7.3, 0.5% BSA, 0.02% sodium azide
Store at -20°C. Minimize freezing and thawing.
For research use only.

Publications (3)

1
Implication of human nonmetastatic clone 23 Type 1 and its downstream gene lipocalin 2 in metastasis and patient's survival of cancer of uterine cervix. Wang PH, Ko JL, Yang SF, Lin LY. International journal of cancer. Journal international du cancer. 2011 Nov;129:2380-9. [Full Text Article] [PubMed:21442621]
2
The role of lipocalin 2 and its concernment with human nonmetastatic clone 23 type 1 and p53 in carcinogenesis of uterine cervix. Wang PH, Yang SF, Tseng CJ, Ying TH, Ko JL, Lin LY. Reproductive sciences (Thousand Oaks, Calif.). 2011 May;18:447-55. [Full Text Article] [PubMed:21273640]
3
Clinical implications of aldo-keto reductase family 1 member C3 and its relationship with lipocalin 2 in cancer of the uterine cervix. Wu CH, Ko JL, Chen SC, Lin YW, Han CP, Yang TY, Chien MH, Wang PH. Gynecologic oncology. 2014 Feb;132:474-82. [Full Text Article] [PubMed:24316309]

About LCN2 / Lipocalin 2 / NGAL

P80188 NM_005564 NP_005555.2

LCN2 Antibody, 24p3 Antibody, HNL Antibody, Lipocalin 2 Antibody, Lipocalin-2 Antibody, MSFI Antibody, Siderocalin LCN2 Antibody, NGAL Antibody, p25 Antibody, Lipocalin 2 (oncogene 24p3) Antibody, Oncogene 24p3 Antibody

Iron-trafficking protein involved in multiple processes such as apoptosis, innate immunity and renal development. Binds iron through association with 2,5-dihydroxybenzoic acid (2,5-DHBA), a siderophore that shares structural similarities with bacterial enterobactin, and delivers or removes iron from the cell, depending on the context.

Immunohistochemistry

Immunohistochemistry
Anti-LCN2 antibody IHC of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B2496 concentration 5 ug/ml.

Western blot

Western blot
Antibody (0.03 ug/ml) staining of Human Kidney lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Requested From: United States
Date Requested: 12/5/2016

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