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Anti-LAMP2 / CD107b Antibody (aa29-375) LS-C149987

Ordering

Wt. Vol. Conc. Price
100 µg - - $395
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)
206-374-1102
866-206-6909
Orders@LSBio.com
 

Most Popular LAMP2 / CD107b Antibodies

Anti-LAMP2 / CD107b Antibody (Internal) IHC-plus™ LS-B581
Rabbit Polyclonal to Human LAMP2 / CD107b
Human, Mouse
IHC - Paraffin, ICC, Western blot
Unconjugated
Immunohistochemistry Image
Anti-LAMP2 / CD107b Antibody IHC-plus™ LS-B3144
Mouse Monoclonal (IgG1) to Dog LAMP2 / CD107b
Dog, Human
IHC - Paraffin, Immunofluorescence, Western blot, Immunoprecipitation, Flow Cytometry
Unconjugated
Immunohistochemistry Image

100% Guaranteed 100% Guaranteed
Goat Polyclonal (IgG) to Human LAMP2 / CD107b
Human
ICC, Western blot, Flow Cytometry
Unconjugated

Details

Human LAMP2 / CD107b
Goat
Human (tested or 100% immunogen sequence identity)
IgG Polyclonal
Unconjugated
Immunoaffinity purified
Unmodified

Applications

  • ICC (5 - 15 µg/ml)
  • Western blot (1 µg/ml)
  • Flow Cytometry

Specificity and Use

LAMP2 / CD107b antibody was raised against mouse myeloma cell line NS0-derived recombinant human LAMP2/CD107b Leu29-Phe375 Accession # P13473.
aa29-375
Detects human LAMP2/CD107b in direct ELISAs and Western blots. In direct ELISAs, less than 1% cross-reactivity with recombinant human.
Western Blot, 1 µg/ml. Immunocytochemistry, 5-15 µg/ml, Intracellular Staining by Flow Cytometry, 2.5 µg/106 cells.

Packaging

Lyophilized from 0.2 um filtered solution in PBS, trehalose
Sterile PBS. Resuspend to 500 ul
Long term: -20°C; Short term: +4°C; Avoid freeze-thaw cycles.
For research use only.

About LAMP2 / CD107b

P13473 NM_013995 NP_054701.1

LAMP2 Antibody, CD107b Antibody, CD107b antigen Antibody, LAMPB Antibody, LGP110 Antibody, LAMP-2 Antibody

Implicated in tumor cell metastasis. May function in protection of the lysosomal membrane from autodigestion, maintenance of the acidic environment of the lysosome, adhesion when expressed on the cell surface (plasma membrane), and inter- and intracellular signal transduction. Protects cells from the toxic effects of methylating mutagens.

Immunocytochemistry

Immunocytochemistry
LAMP2/CD107b in HeLa Human Cell Line. LAMP2/CD107b was detected in immersion fixed HeLa human cervical epithelial carcinoma cell line using Goat Anti-Human LAMP2/CD107b Antigen Affinity-purified Polyclonal Antibody at 15 ug/ml for 3 hours at room temperature. Cells were stained using NorthernLights 557-conjugated Anti-Goat IgG Secondary Antibody (red) and counterstained with DAPI (blue). Specific staining was localized to lysosomes.

Western blot

Western blot
Detection of Human LAMP2/CD107b by Western Blot. Western blot shows lysates of JAR human choriocarcinoma cell line, JEG-3 human epithelial choriocarcinoma cell line, and human placenta tissue. PVDF membrane was probed with 1 ug/ml of Goat Anti-Human LAMP2/CD107b Antigen Affinity-purified Polyclonal Antibody followed by HRP-conjugated Anti-Goat IgG Secondary Antibody. Specific bands were detected for LAMP2/CD107b at approximately 100-120 kD (as indicated). This experiment was conducted under reducing conditions.

Flow Cytometry

Flow Cytometry
Detection of LAMP-2A in HeLa Human Cell Line by Flow Cytometry. HeLa human cervical epithelial carcinoma cell line was stained with Goat Anti-Human LAMP2/CD107b Antigen Affinity-purified Polyclonal Antibody or control antibody, followed by Allophycocyanin-conjugated Anti-Goat IgG Secondary Antibody. To facilitate intracellular staining, cells were fixed with paraformaldehyde and permeabilized with saponin.
 

Requested From: United States
Date Requested: 12/5/2016

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