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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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LAMP2 / CD107b Antibody (clone H4B4) LS-C359342

CD107b antibody LS-C359342 is an unconjugated mouse monoclonal antibody to human CD107b (LAMP2). Validated for Flow, ICC, IF, IHC and WB.
Catalog
Size
Price
LS-C359342-100
100 µl (1 mg/ml)
$335
Description
CD107b antibody LS-C359342 is an unconjugated mouse monoclonal antibody to human CD107b (LAMP2). Validated for Flow, ICC, IF, IHC and WB.
Target
Human LAMP2 / CD107b
Host
Mouse
Reactivity
Human (tested or 100% immunogen sequence identity)
Non-Reactivity
Mouse, Rat
Clonality
IgG1,k Monoclonal
Clone
H4B4
Conjugations
Unconjugated
Purification
Protein G purified
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin (1:100 - 1:200)
  • IHC - Frozen (1:100 - 1:200)
  • ICC (1:10 - 1:100)
  • Immunofluorescence (1:10 - 1:100)
  • Western blot (1:25)
  • Flow Cytometry (1 µg/10E6 cells)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
LAMP2 / CD107b antibody was raised against human adherent spleen cells.
Specificity
Human LAMP2 / CD107b.
Usage
In Western blot, bands may be seen at ~40 kDa and 45 kDa representing the unglycosylated isoforms of LAMP2 and ~110 kDa representing the glycosylated form.In Simple Western only 10-15 uL of the recommended dilution is used per data point.
Presentation
PBS, 0.05% Sodium Azide
Storage
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Restrictions
For research use only.
About LAMP2 / CD107b
P13473 NM_013995 NP_054701.1

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Images

Immunohistochemistry

Immunohistochemistry-Paraffin: LAMP2 Antibody (H4B4) - IHC staining of LAMP2 in human liver using DAB with hematoxylin counterstain.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Immunohistochemistry-Paraffin: LAMP2 Antibody (H4B4) - IHC staining of LAMP2 in human liver using DAB with hematoxylin counterstain. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Immunofluorescence

Immunocytochemistry/Immunofluorescence: LAMP2 Antibody (H4B4) - The LAMP2 antibody was tested in HeLa cells at a 1:10 dilution against Dylight 488 (Green). Actin was counterstained against Phalloidin 568 (Red) and cells were mounted in DAPI Flouromount (Blue).  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Immunocytochemistry/Immunofluorescence: LAMP2 Antibody (H4B4) - The LAMP2 antibody was tested in HeLa cells at a 1:10 dilution against Dylight 488 (Green). Actin was counterstained against Phalloidin 568 (Red) and cells were mounted in DAPI Flouromount (Blue). This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

Western Blot: LAMP-2/CD107b Antibody (H4B4) - Western blot shows a specific band for LAMP2 in 1.0 mg/ml of HeLa lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Western Blot: LAMP-2/CD107b Antibody (H4B4) - Western blot shows a specific band for LAMP2 in 1.0 mg/ml of HeLa lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

Western Blot: LAMP2 Antibody (H4B4) - Analysis of LAMP2 expression in HeLa, Jurkat, U937, HepG2 whole cell lysates, human kidney, lung, liver and placenta tissue extracts.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Western Blot: LAMP2 Antibody (H4B4) - Analysis of LAMP2 expression in HeLa, Jurkat, U937, HepG2 whole cell lysates, human kidney, lung, liver and placenta tissue extracts. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Flow Cytometry

Flow Cytometry: LAMP2 Antibody (H4B4) - Intracellular flow cytometric staining of 1 x 10^6 HEK-293 cells using LAMP2 antibody (dark blue). Isotype control shown in orange. An antibody concentration of 1 ug/1x10^6 cells was used.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Flow Cytometry: LAMP2 Antibody (H4B4) - Intracellular flow cytometric staining of 1 x 10^6 HEK-293 cells using LAMP2 antibody (dark blue). Isotype control shown in orange. An antibody concentration of 1 ug/1x10^6 cells was used. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Immunohistochemistry

Immunohistochemistry-Paraffin: LAMP2 Antibody (H4B4) - IHC staining of LAMP2 in human liver using DAB with hematoxylin counterstain.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Immunohistochemistry-Paraffin: LAMP2 Antibody (H4B4) - IHC staining of LAMP2 in human liver using DAB with hematoxylin counterstain. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Immunofluorescence

Immunocytochemistry/Immunofluorescence: LAMP2 Antibody (H4B4) - The LAMP2 antibody was tested in HeLa cells at a 1:10 dilution against Dylight 488 (Green). Actin was counterstained against Phalloidin 568 (Red) and cells were mounted in DAPI Flouromount (Blue).  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Immunocytochemistry/Immunofluorescence: LAMP2 Antibody (H4B4) - The LAMP2 antibody was tested in HeLa cells at a 1:10 dilution against Dylight 488 (Green). Actin was counterstained against Phalloidin 568 (Red) and cells were mounted in DAPI Flouromount (Blue). This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

Western Blot: LAMP-2/CD107b Antibody (H4B4) - Western blot shows a specific band for LAMP2 in 1.0 mg/ml of HeLa lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Western Blot: LAMP-2/CD107b Antibody (H4B4) - Western blot shows a specific band for LAMP2 in 1.0 mg/ml of HeLa lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

Western Blot: LAMP2 Antibody (H4B4) - Analysis of LAMP2 expression in HeLa, Jurkat, U937, HepG2 whole cell lysates, human kidney, lung, liver and placenta tissue extracts.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Western Blot: LAMP2 Antibody (H4B4) - Analysis of LAMP2 expression in HeLa, Jurkat, U937, HepG2 whole cell lysates, human kidney, lung, liver and placenta tissue extracts. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Flow Cytometry

Flow Cytometry: LAMP2 Antibody (H4B4) - Intracellular flow cytometric staining of 1 x 10^6 HEK-293 cells using LAMP2 antibody (dark blue). Isotype control shown in orange. An antibody concentration of 1 ug/1x10^6 cells was used.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Flow Cytometry: LAMP2 Antibody (H4B4) - Intracellular flow cytometric staining of 1 x 10^6 HEK-293 cells using LAMP2 antibody (dark blue). Isotype control shown in orange. An antibody concentration of 1 ug/1x10^6 cells was used. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Immunohistochemistry

Immunohistochemistry-Paraffin: LAMP2 Antibody (H4B4) - IHC staining of LAMP2 in human liver using DAB with hematoxylin counterstain.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Immunohistochemistry-Paraffin: LAMP2 Antibody (H4B4) - IHC staining of LAMP2 in human liver using DAB with hematoxylin counterstain. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Immunofluorescence

Immunocytochemistry/Immunofluorescence: LAMP2 Antibody (H4B4) - The LAMP2 antibody was tested in HeLa cells at a 1:10 dilution against Dylight 488 (Green). Actin was counterstained against Phalloidin 568 (Red) and cells were mounted in DAPI Flouromount (Blue).  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Immunocytochemistry/Immunofluorescence: LAMP2 Antibody (H4B4) - The LAMP2 antibody was tested in HeLa cells at a 1:10 dilution against Dylight 488 (Green). Actin was counterstained against Phalloidin 568 (Red) and cells were mounted in DAPI Flouromount (Blue). This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

Western Blot: LAMP-2/CD107b Antibody (H4B4) - Western blot shows a specific band for LAMP2 in 1.0 mg/ml of HeLa lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Western Blot: LAMP-2/CD107b Antibody (H4B4) - Western blot shows a specific band for LAMP2 in 1.0 mg/ml of HeLa lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

Western Blot: LAMP2 Antibody (H4B4) - Analysis of LAMP2 expression in HeLa, Jurkat, U937, HepG2 whole cell lysates, human kidney, lung, liver and placenta tissue extracts.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Western Blot: LAMP2 Antibody (H4B4) - Analysis of LAMP2 expression in HeLa, Jurkat, U937, HepG2 whole cell lysates, human kidney, lung, liver and placenta tissue extracts. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Flow Cytometry

Flow Cytometry: LAMP2 Antibody (H4B4) - Intracellular flow cytometric staining of 1 x 10^6 HEK-293 cells using LAMP2 antibody (dark blue). Isotype control shown in orange. An antibody concentration of 1 ug/1x10^6 cells was used.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Flow Cytometry: LAMP2 Antibody (H4B4) - Intracellular flow cytometric staining of 1 x 10^6 HEK-293 cells using LAMP2 antibody (dark blue). Isotype control shown in orange. An antibody concentration of 1 ug/1x10^6 cells was used. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Immunohistochemistry

Immunohistochemistry-Paraffin: LAMP2 Antibody (H4B4) - IHC staining of LAMP2 in human liver using DAB with hematoxylin counterstain.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Immunohistochemistry-Paraffin: LAMP2 Antibody (H4B4) - IHC staining of LAMP2 in human liver using DAB with hematoxylin counterstain. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Immunofluorescence

Immunocytochemistry/Immunofluorescence: LAMP2 Antibody (H4B4) - The LAMP2 antibody was tested in HeLa cells at a 1:10 dilution against Dylight 488 (Green). Actin was counterstained against Phalloidin 568 (Red) and cells were mounted in DAPI Flouromount (Blue).  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Immunocytochemistry/Immunofluorescence: LAMP2 Antibody (H4B4) - The LAMP2 antibody was tested in HeLa cells at a 1:10 dilution against Dylight 488 (Green). Actin was counterstained against Phalloidin 568 (Red) and cells were mounted in DAPI Flouromount (Blue). This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

Western Blot: LAMP-2/CD107b Antibody (H4B4) - Western blot shows a specific band for LAMP2 in 1.0 mg/ml of HeLa lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Western Blot: LAMP-2/CD107b Antibody (H4B4) - Western blot shows a specific band for LAMP2 in 1.0 mg/ml of HeLa lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

Western Blot: LAMP2 Antibody (H4B4) - Analysis of LAMP2 expression in HeLa, Jurkat, U937, HepG2 whole cell lysates, human kidney, lung, liver and placenta tissue extracts.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Western Blot: LAMP2 Antibody (H4B4) - Analysis of LAMP2 expression in HeLa, Jurkat, U937, HepG2 whole cell lysates, human kidney, lung, liver and placenta tissue extracts. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Flow Cytometry

Flow Cytometry: LAMP2 Antibody (H4B4) - Intracellular flow cytometric staining of 1 x 10^6 HEK-293 cells using LAMP2 antibody (dark blue). Isotype control shown in orange. An antibody concentration of 1 ug/1x10^6 cells was used.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Flow Cytometry: LAMP2 Antibody (H4B4) - Intracellular flow cytometric staining of 1 x 10^6 HEK-293 cells using LAMP2 antibody (dark blue). Isotype control shown in orange. An antibody concentration of 1 ug/1x10^6 cells was used. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Requested From: United States
Date Requested: 3/21/2019
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