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Anti-KLHL22 Antibody (clone 7B10) LS-C169259

Note: This antibody replaces LS-C171516

Ordering

Wt. Vol. Conc. Price
- 100 µl - $345
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)
206-374-1102
866-206-6909
Orders@LSBio.com
 

Most Popular KLHL22 Antibodies

Anti-KLHL22 Antibody (clone 7B10) LS-C169259
Mouse Monoclonal [clone 7B10] (IgG1) to Human KLHL22
Human
ICC, Immunofluorescence, Western blot, ELISA
Unconjugated
Immunofluorescence Image
Anti-KLHL22 Antibody (aa586-614, AP) LS-C261435
Rabbit Polyclonal (IgG) to Human KLHL22
Human, Mouse
Western blot, ELISA
AP Conjugated
Anti-KLHL22 Antibody (aa586-614, Azide-free, HRP) LS-C261441
Rabbit Polyclonal (IgG) to Human KLHL22
Human, Mouse
Western blot, ELISA
HRP Conjugated, Azide-free

100% Guaranteed 100% Guaranteed
Mouse Monoclonal [clone 7B10] (IgG1) to Human KLHL22
Human
ICC, Immunofluorescence, Western blot, ELISA
Unconjugated

Details

Human KLHL22
Mouse
Human (tested or 100% immunogen sequence identity)
IgG1 Monoclonal [7B10]
Unconjugated
Ascites
Unmodified

Applications

  • ICC (1:200 - 1:1000)
  • Immunofluorescence (1:1000)
  • Western blot (1:500 - 1:2000)
  • ELISA (1:10000)

Specificity and Use

KLHL22 antibody was raised against purified recombinant fragment of human KLHL22 expressed in E. coli.
Human KLHL22

Packaging

Ascites, 0.03% sodium azide
Long term: -20°C; Short term: +4°C; Avoid freeze-thaw cycles.
For research use only.

About KLHL22

Q53GT1 NM_032775 NP_116164.2

KLHL22 Antibody, Kelch-like 22 (Drosophila) Antibody, Kelch-like protein 22 Antibody, KELCHL Antibody

Substrate-specific adapter of a BCR (BTB-CUL3-RBX1) E3 ubiquitin ligase complex required for chromosome alignment and localization of PLK1 at kinetochores. The BCR(KLHL22) ubiquitin ligase complex mediates monoubiquitination of PLK1, leading to PLK1 dissociation from phosphoreceptor proteins and subsequent removal from kinetochores, allowing silencing of the spindle assembly checkpoint (SAC) and chromosome segregation. Monoubiquitination of PLK1 does not lead to PLK1 degradation.

Immunofluorescence

Immunofluorescence
Immunofluorescence of U251 cells using KLHL22 mouse monoclonal antibody (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.

Western blot

Western blot
Western blot using KLHL22 mouse monoclonal antibody against mouse brain tissues lysate.

Requested From: United States
Date Requested: 12/9/2016

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