Target
Human KDR / VEGFR2 / FLK1
Synonyms
KDR | CD309 | Fetal liver kinase 1 | Fetal liver kinase-1 | FLK-1 | FLK1 | Kinase insert domain receptor | Quek1 | VEGFR | VEGFR2 | Soluble VEGFR2 | VEGF receptor 2 | VEGFR-2 | CD309 antigen | VEGF-R2
Reactivity
Human, Mouse
(tested or 100% immunogen sequence identity)
Conjugations
Unconjugated
Purification
Immunoaffinity purified
Immunogen
Synthetic phospho-peptide (KLH coupled) corresponding to residues surrounding Tyr951 of human VEGF receptor 2.
Specificity
Detects overexpressed human VEGFR-2 only when phosphorylated at tyrosine 951. Does not cross-react with other phosphorylated receptor tyrosine kinases. Species cross-reactivity: Predicted to cross-react with mouse based on 100% sequence homology.
Applications
- Western blot (1:1000)
- Immunoprecipitation (1:50)
Usage
Suitable for use in Western Blot and Immunoprecipitation. Western Blot: 1:1000, incubate membrane with diluted antibody in 5% BSA, 1X TBS, 0.1% Tween-20 at 4?C with gentle shaking, overnight. Immunoprecipitation: 1:50.
Presentation
10 mM HEPES, pH 7.5, 150 mM NaCl, 0.1 mg/ml BSA, 50% Glycerol, No preservatives added
Storage
Short term: store at 4°C. Long term: store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About KDR / VEGFR2 / FLK1