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| Catalog Number | Size | Price |
|---|---|---|
| LS-A9456-50 | 50 µg (0.4 mg/ml) | $440 |
IHC‑plus™ Polyclonal Rabbit anti‑Human KDR / VEGFR2 / FLK1 Antibody (Internal, IHC) LS‑A9456
IHC‑plus™ Polyclonal Rabbit anti‑Human KDR / VEGFR2 / FLK1 Antibody (Internal, IHC) LS‑A9456
Note: This antibody replaces LS-C92355
Antibody:
KDR / VEGFR2 / FLK1 Rabbit anti-Human Polyclonal (Internal) Antibody
Application:
IHC, IHC-P
Reactivity:
Human, Monkey, Dog
Format:
Unconjugated, Unmodified
Overview
Antibody:
KDR / VEGFR2 / FLK1 Rabbit anti-Human Polyclonal (Internal) Antibody
Application:
IHC, IHC-P
Reactivity:
Human, Monkey, Dog
Format:
Unconjugated, Unmodified
Specifications
Description
FLK1 antibody LS-A9456 is an unconjugated rabbit polyclonal antibody to FLK1 (KDR / VEGFR2) (Internal) from human. It is reactive with human, dog and monkey. Validated for IHC. Tested on 20 paraffin-embedded human tissues.
Target
Human KDR / VEGFR2 / FLK1
Synonyms
KDR | CD309 | Fetal liver kinase 1 | Fetal liver kinase-1 | FLK-1 | FLK1 | Kinase insert domain receptor | Quek1 | VEGFR | VEGFR2 | Soluble VEGFR2 | VEGF receptor 2 | VEGFR-2 | CD309 antigen | VEGF-R2
Host
Rabbit
Reactivity
Human, Monkey, Dog
(tested or 100% immunogen sequence identity)
Predicted
Bat, Bovine, Hamster, Horse (at least 90% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Immunoaffinity purified
Modifications
Unmodified
Immunogen
Synthetic 15 amino acid peptide from internal region of human VEGFR2. Percent identity with other species by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset, Dog (100%); Bovine, Bat, Hamster, Panda, Horse (93%); Mouse, Rat, Pig, Platypus (87%); Elephant, Opossum (80%).
Epitope
Internal
Specificity
Human VEGFR2. BLAST analysis of the peptide immunogen showed no homology with other human proteins, except SLC22A7 (60%).
Applications
- IHC
- IHC - Paraffin (2.5 µg/ml)
|
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents
ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
|
Usage
Immunohistochemistry: The unconjugated form was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for the unconjugated form was determined to be 2.5 ug/ml.
Presentation
PBS, 0.1% Sodium Azide
Storage
Aliquot and store undiluted at -20°C or below for up to 1 year. Can be stored undiluted at 4°C for up to 1 month. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
About KDR / VEGFR2 / FLK1
Validation
Anti-KDR / VEGFR2 antibody IHC of human Breast, Carcinoma. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
Anti-KDR / VEGFR2 antibody IHC of human Breast, Carcinoma. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
See More About...
LSBio Ratings
IHC-plus™ KDR / VEGFR2 / FLK1 Antibody (Internal) for IHC LS-A9456 has an LSBio Rating of
Laboratory Validation Score (4)
Learn more about The LSBio Ratings Algorithm
Publications (0)
Customer Reviews (0)
Featured Products
Species:
Human
Applications:
IHC, IHC - Paraffin
Species:
Human
Applications:
IHC, IHC - Paraffin, IHC - Frozen, Western blot
Species:
Human
Applications:
Immunofluorescence, Western blot, Flow Cytometry, ELISA
Species:
Mouse
Applications:
IHC, IHC - Paraffin, Western blot, ELISA
Reactivity:
Human, Mouse, Rat
Range:
Positive/Negative
Request SDS/MSDS
To request an SDS/MSDS form for this product, please contact our Technical Support department at:
Technical.Support@LSBio.com
Requested From: United States
Date Requested: 4/19/2024
Date Requested: 4/19/2024
