Human KDR / VEGFR2 / FLK1

Rabbit

Human, Mouse (tested or 100% immunogen sequence identity)

Polyclonal

Unconjugated

Immunoaffinity purified

Unmodified

Chemically synthesized phosphopeptide derived from the region of human VEGFR2 that contains tyrosine 1054. The sequence is conserved in mouse and rat.

pTyr1054

Mouse VEGFR2. Human and rat (100% homologous) VEGFR2 have not been tested, but are expected to react. VEGFR3 (100%) has not been tested, but is expected to react.

• Western blot

Suitable for use in Western blotting applications. For Western blotting applications, we recommend using the antibody at 0.35-1.0 ug/ml. At 0.50 ug/ml, the dilution provides 100 ml working solution, which at 10 ml/blot allows 10 blots to be performed. Positive control: NIH3T3 cells transfected with full-length, wild type VEGFR2, or Porcine Aortic Epithelial (PAE) cells transfected with a chimeric receptor consisting of the extracellular domain of the CSF-1 receptor coupled to the transmembrane and cytoplasmic domains of the VEGFR2.

PBS (without Mg2+, Ca2+), 1 mg/ml BSA (IgG, protease free), pH 7.2, 0.1% sodium azide.

Short term: store at 4°C. Long term: store at -20°C. Avoid freeze-thaw cycles.

For research use only. Intended for use by laboratory professionals.

This antibody carries the LSBio 100% Guarantee.

Uniprot: P35968 NCBI: NM_002253 NP_002244.1