Target
Human KDR / VEGFR2 / FLK1
Synonyms
KDR | CD309 | Fetal liver kinase 1 | Fetal liver kinase-1 | FLK-1 | FLK1 | Kinase insert domain receptor | Quek1 | VEGFR | VEGFR2 | Soluble VEGFR2 | VEGF receptor 2 | VEGFR-2 | CD309 antigen | VEGF-R2
Reactivity
Human, Mouse
(tested or 100% immunogen sequence identity)
Conjugations
Unconjugated
Purification
Immunoaffinity purified
Immunogen
Chemically synthesized phosphopeptide derived from the region of human VEGFR2 that contains tyrosine 1054. The sequence is conserved in mouse and rat.
Specificity
Mouse VEGFR2. Human and rat (100% homologous) VEGFR2 have not been tested, but are expected to react. VEGFR3 (100%) has not been tested, but is expected to react.
Usage
Suitable for use in Western blotting applications. For Western blotting applications, we recommend using the antibody at 0.35-1.0 ug/ml. At 0.50 ug/ml, the dilution provides 100 ml working solution, which at 10 ml/blot allows 10 blots to be performed. Positive control: NIH3T3 cells transfected with full-length, wild type VEGFR2, or Porcine Aortic Epithelial (PAE) cells transfected with a chimeric receptor consisting of the extracellular domain of the CSF-1 receptor coupled to the transmembrane and cytoplasmic domains of the VEGFR2.
Presentation
PBS (without Mg2+, Ca2+), 1 mg/ml BSA (IgG, protease free), pH 7.2, 0.1% sodium azide.
Storage
Short term: store at 4°C. Long term: store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About KDR / VEGFR2 / FLK1