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Anti-KDR / VEGFR2 / FLK1 Antibody (aa20-764) LS-C150097

Ordering

Wt. Vol. Conc. Price
100 µg - - $395
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)
206-374-1102
866-206-6909
Orders@LSBio.com
 

Most Popular KDR / VEGFR2 / FLK1 Antibodies

Anti-KDR / VEGFR2 / FLK1 Antibody (Internal) IHC-plus™ LS-A9456
Rabbit Polyclonal to Human KDR / VEGFR2 / FLK1
Human, Monkey, Dog
IHC - Paraffin
Unconjugated
Immunohistochemistry Image
Anti-KDR / VEGFR2 / FLK1 Antibody (aa1300-1367) IHC-plus™ LS-B669
Rabbit Polyclonal to Mouse KDR / VEGFR2 / FLK1
Mouse, Human
IHC - Paraffin, ICC, Immunofluorescence, Western blot
Unconjugated
Immunohistochemistry Image
Anti-KDR / VEGFR2 / FLK1 Antibody (Extracellular Domain) LS-C122532
Mouse Monoclonal (IgG1) to Human KDR / VEGFR2 / FLK1
Human
IHC - Paraffin, IHC - Frozen, Western blot
Unconjugated

100% Guaranteed 100% Guaranteed
Goat Polyclonal (IgG) to Human KDR / VEGFR2 / FLK1
Human
IHC - Paraffin, Western blot, Neutralization
Unconjugated

Details

Human KDR / VEGFR2 / FLK1
Goat
Human (tested or 100% immunogen sequence identity)
IgG Polyclonal
Unconjugated
Immunoaffinity purified
Unmodified

Applications

  • IHC - Paraffin (5 - 15 µg/ml)
  • Western blot (0.1 µg/ml)
  • Neutralization

Specificity and Use

KDR / VEGFR2 / FLK1 antibody was raised against mouse myeloma cell line NS0-derived recombinant human VEGF R2/KDR/Flk1 Ala20-Glu764 Accession # AAC16450.
aa20-764
Detects human VEGF R2/KDR/Flk-1 in direct ELISAs and Western blots. In direct ELISAs, approximately 40% cross-reactivity with.
Western Blot, 0.1 µg/ml, Recombinant Human VEGF R2/KDR/Flk-1 Fc Chimera. Immunohistochemistry, 5-15 µg/ml. Neutralization, Measured by its ability to neutralize VEGF R2/KDR/Flk-1-mediated inhibition of proliferation in HUVEC human. umbilical vein endothelial cells. The Neutralization Dose (ND50) is typically 0.05-0.25 µg/ml in the presence of. 30 ng/ml Recombinant Human VEGF R2/KDR/Flk-1 Fc Chimera and 5 ng/ml Recombinant Human VEGF165.

Packaging

Lyophilized from 0.2 um filtered solution in PBS, trehalose
Sterile PBS. Resuspend to 500 ul
Store at -20 to -70 C. Stable for 12 months from date of receipt.
For research use only.

About KDR / VEGFR2 / FLK1

P35968 NM_002253 NP_002244.1

KDR Antibody, CD309 Antibody, Fetal liver kinase 1 Antibody, Fetal liver kinase-1 Antibody, FLK-1 Antibody, FLK1 Antibody, Kinase insert domain receptor Antibody, Quek1 Antibody, VEGFR Antibody, VEGFR2 Antibody, Soluble VEGFR2 Antibody, VEGF receptor 2 Antibody, VEGFR-2 Antibody, CD309 antigen Antibody, VEGF-R2 Antibody

Tyrosine-protein kinase that acts as a cell-surface receptor for VEGFA, VEGFC and VEGFD. Plays an essential role in the regulation of angiogenesis, vascular development, vascular permeability, and embryonic hematopoiesis. Promotes proliferation, survival, migration and differentiation of endothelial cells. Promotes reorganization of the actin cytoskeleton. Isoforms lacking a transmembrane domain, such as isoform 2 and isoform 3, may function as decoy receptors for VEGFA, VEGFC and/or VEGFD.

Immunohistochemistry

Immunohistochemistry
VEGF R2/KDR/Flk-1 in Human Kidney. VEGFR2/KDR/Flk-1 was detected in immersion fixed paraffin-embedded sections of human kidney using Goat Anti-Human VEGF R2/KDR/Flk-1 Antigen Affinity-purified Polyclonal Antibody at 10 ug/ml overnight at 4C. Tissue was stained using Anti-Goat HRP-DAB (brown) and counterstained with hematoxylin (blue). Lower panel shows a lack of labeling if primary antibodies are omitted and tissue is stained only with secondary antibody followed by incubation with detection reagents. View our protocol for Chromogenic IHC of Paraffin-embedded Tissue Sections.

Immunohistochemistry

Immunohistochemistry
VEGF R2/KDR/Flk-1 in Human Placenta. VEGF R2/KDR/Flk-1 was detected in immersion fixed paraffin-embedded sections of human placenta using 15 ug/ml Goat Anti-Human VEGF R2/ KDR/Flk-1 Antigen Affinity-purified Polyclonal Antibody overnight at 4°C. Tissue was stained with Anti-Goat HRP-AEC (red) and counterstained with hematoxylin (blue).

Neutralization

Neutralization
VEGF R2/KDR/Flk-1 Inhibition of VEGF-dependent Cell Proliferation and Neutralization by Human VEGF R2/KDR/Flk-1 Antibody. Recombinant Human VEGFR2/KDR/Flk-1 Fc Chimera inhibits Recombinant Human VEGF165 induced proliferation in HUVEC human umbilical vein endothelial cells in a dose-dependent manner (orange line). Inhibition of Recombinant Human VEGF165 (5 ng/ml) activity elicited by Recombinant Human VEGFR2/KDR/Flk-1 Fc Chimera (30 ng/ml) is neutralized (green line) by increasing concentrations of Human VEGFR2/KDR/Flk-1 Antigen Affinity-purified Polyclonal Antibody. The ND50 is typically 0.05-0.25 ug/ml.
 

Requested From: United States
Date Requested: 12/4/2016

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