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Anti-KDM1A / LSD1 Antibody (aa800-850) IHC-plus™ LS-B1777

Note: This antibody replaces LS-C47286

Ordering

Wt. Vol. Conc. Price
50 µg - 0.5 mg/ml $395
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)
206-374-1102
866-206-6909
Orders@LSBio.com
 

Most Popular KDM1A / LSD1 Antibodies

Anti-KDM1A / LSD1 Antibody (aa100-150) IHC-plus™ LS-B2014
Rabbit Polyclonal to Human KDM1A / LSD1
Human, Monkey, Mouse, Rat, Dog, Zebrafish, Macaque
IHC - Paraffin, Western blot
Unconjugated
Immunohistochemistry Image
Anti-KDM1A / LSD1 Antibody (clone 2E7) IHC-plus™ LS-B6214
Mouse Monoclonal [clone 2E7] (IgG2a,k) to Human KDM1A / LSD1
Human
IHC - Paraffin, Immunofluorescence, Western blot, ELISA
Unconjugated
Immunohistochemistry Image

100% Guaranteed 100% Guaranteed
Rabbit Polyclonal (IgG) to Human KDM1A / LSD1
Human, Monkey, Mouse, Rat, Dog
IHC - Paraffin, Western blot
Unconjugated

Details

Human KDM1A / LSD1
Rabbit
Human, Monkey, Mouse, Rat, Dog (tested or 100% immunogen sequence identity)
IgG Polyclonal
Unconjugated
Protein G purified
Unmodified

Applications

  • IHC - Paraffin (10 µg/ml)
  • Western blot (1 - 3 µg/ml)

Specificity and Use

KDM1A / LSD1 antibody was raised against a portion of amino acids 800-850 of human LSD1.
aa800-850
The amino acid sequence used as immunogen is 100% homologous in human (isoforms CRA_a, CRA _b and CRA _c), rhesus monkey, dog (isoforms 1 and 2), mouse (isoforms 1, 2, 3 and 4) and rat.
Immunohistochemistry: LS-B1777 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B1777 was determined to be 10 ug/ml.

Packaging

PBS, 0.2% gelatin, 0.05% sodium azide.
Short term 4°C, long term aliquot and store at -20°C, avoid freeze thaw cycles.
For research use only.

About KDM1A / LSD1

O60341 NM_015013 NP_055828.2

KDM1A Antibody, BHC110 Antibody, KDM1 Antibody, LSD1 Antibody, AOF2 Antibody, KIAA0601 Antibody

Histone demethylase that demethylates both 'Lys-4' (H3K4me) and 'Lys-9' (H3K9me) of histone H3, thereby acting as a coactivator or a corepressor, depending on the context. Acts by oxidizing the substrate by FAD to generate the corresponding imine that is subsequently hydrolyzed. Acts as a corepressor by mediating demethylation of H3K4me, a specific tag for epigenetic transcriptional activation. Demethylates both mono- (H3K4me1) and di-methylated (H3K4me2) H3K4me.

Immunohistochemistry

Immunohistochemistry
Anti-KDM1A / LSD1 antibody IHC of human brain, cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B1777 concentration 10 ug/ml.

Western blot

Western blot
Western blot ofulSD1 in Jurkat cell lysate in the 1) absence and 2) presence of immunizing peptide using antibody at 1 ug/ml.

Requested From: United States
Date Requested: 12/4/2016

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