Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to
worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting
immunostaining in relation to complex human pathologies.
TCR Screening Services
Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding.
Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".
(applications tested for the base form of this product only)
Specificity and Use
KCNH5 / Kv10.2 antibody was raised against synthetic peptide located between aa156-205 of human KCNH5 (Q8NCM2-2, NP_758963). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Monkey, Galago, Marmoset, Mouse, Rat, Elephant, Panda, Dog, Bovine, Bat, Rabbit, Horse, Pig, Opossum, Guinea pig, Turkey, Zebra finch, Chicken, Platypus, Lizard, Xenopus, Zebrafish (100%).
LS-E12618 - Lyophilized - 100 µg - $145.00
Immunizing peptide used to generate LS-B975. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
Human KCNH5 / Kv10.2
Immunohistochemistry: LS-B975 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B975 was determined to be 5 ug/ml.
Lyophilized from PBS with 2% sucrose
Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year); Short term (less than one week): +4°C. Avoid repeat freeze-thaw cycles.
Anti-KCNH5 / Kv10.2 antibody IHC of human heart. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B975 concentration 5 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested.
KCNH5 / Kv10.2 antibody LS-B975 Western blot of HepG2 cell lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.
HepG2 Cell Lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.