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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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IVL / Involucrin Antibody (clone IVRN/827, Azide‑free) LS‑C390475
Involucrin antibody LS-C390475 is an unconjugated mouse monoclonal antibody to human Involucrin (IVL). Validated for Flow, IF and IHC.
Catalog
Size
Price
LS-C390475-100
100 µg (1 mg/ml)
$415

Popular IVL / Involucrin Products

Anti-IVL / Involucrin antibody IHC of human tonsil. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B3058 concentration 10 ug/ml.
Species: Human
Applications: IHC, IHC - Paraffin, Western blot
Anti-IVL / Involucrin antibody IHC of human skin. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B6298 concentration 10 ug/ml.
Species: Human, Monkey, Dog, Pig
Applications: IHC, IHC - Paraffin, IHC - Frozen, Western blot, Immunoprecipitation, ELISA
Antibody
Species: Human, Dog, Pig
Applications: IHC, IHC - Paraffin, IHC - Frozen, ICC, Western blot
Western blot of recombinant IVL / Involucrin.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Species: Mouse
Applications: IHC, Western blot
Antibody
Species: Human, Gorilla, Dog, Pig, Owl monkey
Applications: IHC, IHC - Paraffin, Immunofluorescence, Flow Cytometry

Product Description

Involucrin antibody LS-C390475 is an unconjugated mouse monoclonal antibody to human Involucrin (IVL). Validated for Flow, IF and IHC.
About IVL / Involucrin
Part of the insoluble cornified cell envelope (CE) of stratified squamous epithelia. P07476 NM_005547 NP_005538.2

IVL Antibody, Involucrin Antibody


Specifications

Target
Human IVL / Involucrin
Host
Mouse
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
IgG1,k Monoclonal [IVRN/827]
Conjugations
Unconjugated
Purification
Protein G purified
Modifications
Azide-free. Also available Unmodified or Ready-to-Use.
Applications
  • IHC
  • IHC - Paraffin (0.1 - 0.2 µg/ml)
  • Immunofluorescence (1 - 2 µg/ml)
  • Flow Cytometry (0.5 - 1 µg/10E6 cells)
  • (applications tested for the base form of this product only)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
Purified Involucrin from human keratinocytes.
Specificity
Human IVL / Involucrin
Usage
The applications listed have been tested for the unmodified form of this product. Other forms have not been tested. It recognizes a protein of 66kDa-170kDa, identified as involucrin. In Western blotting of cultured human keratinocytes, this mAb reacts with a 120kDa protein. Involucrin is expressed in a range of stratified squamous epithelia, including the cornea, which lacks a distinct cornified layer. In normal epidermis, it is first expressed in the upper spinous layers, and in keratinocyte cultures, all cells that have left the basal layer express it. Involucrin expression is altered in pathological conditions: in psoriasis and other benign epidermal hyperplasias, involucrin expression begins closer to the basal layer than normal. expression is abnormal in squamous cell carcinomas and premalignant lesions, and is reduced in severe dysplasias of the larynx and cervix. Optimal dilution of the Involucrin antibody should be determined by the researcher. Staining of formalin-fixed tissues is enhanced by digestion with Trypsin or Protease XXV at 1mg/ml PBS for 5 min at 37oC. Enzyme digestion is more effective than Citrate buffer based epitope unmasking.
Presentation
Sterile PBS.
Storage
Store at 2°C to 8°C.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.

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Images

Immunohistochemistry

Formalin-fixed, paraffin-embedded human tonsil stained with Involucrin antibody (IVRN/827).  This image was taken for the unmodified form of this product. Other forms have not been tested.
Formalin-fixed, paraffin-embedded human tonsil stained with Involucrin antibody (IVRN/827). This image was taken for the unmodified form of this product. Other forms have not been tested.

Immunohistochemistry

Formalin-fixed, paraffin-embedded human tonsil stained with Involucrin antibody (IVRN/827).  This image was taken for the unmodified form of this product. Other forms have not been tested.
Formalin-fixed, paraffin-embedded human tonsil stained with Involucrin antibody (IVRN/827). This image was taken for the unmodified form of this product. Other forms have not been tested.

Immunohistochemistry

Formalin-fixed, paraffin-embedded human tonsil stained with Involucrin antibody (IVRN/827).  This image was taken for the unmodified form of this product. Other forms have not been tested.
Formalin-fixed, paraffin-embedded human tonsil stained with Involucrin antibody (IVRN/827). This image was taken for the unmodified form of this product. Other forms have not been tested.

Immunohistochemistry

Formalin-fixed, paraffin-embedded human tonsil stained with Involucrin antibody (IVRN/827).  This image was taken for the unmodified form of this product. Other forms have not been tested.
Formalin-fixed, paraffin-embedded human tonsil stained with Involucrin antibody (IVRN/827). This image was taken for the unmodified form of this product. Other forms have not been tested.

Requested From: United States
Date Requested: 7/22/2018

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