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Anti-IVL / Involucrin Antibody (C-Terminus) LS-C352399

Catalog Size Price
LS-C352399-100 100 µl Unavailable

Most Popular IVL / Involucrin Antibodies

Anti-IVL / Involucrin Antibody (aa420-578) IHC-plus™ LS-B3058
Rabbit Polyclonal to Human IVL / Involucrin
Human
IHC - Paraffin, Western blot
Unconjugated
Immunohistochemistry Image
Anti-IVL / Involucrin Antibody (clone SY5) IHC-plus™ LS-B6298
Mouse Monoclonal [clone SY5] (IgG1) to Human IVL / Involucrin
Human, Monkey, Dog, Pig
IHC - Paraffin, IHC - Frozen, Western blot, Immunoprecipitation, ELISA
Unconjugated
Immunohistochemistry Image
Anti-IVL / Involucrin Antibody (clone IL-9) LS-C171169
Mouse Monoclonal [clone IL-9] (IgG1) to Human IVL / Involucrin
Human, Dog, Pig
IHC - Paraffin, IHC - Frozen, ICC, Western blot
Unconjugated
Anti-IVL / Involucrin Antibody (aa301-460) LS-C294948
Rabbit Polyclonal (IgG) to Mouse IVL / Involucrin
Mouse
Western blot, ELISA
Unconjugated
Western blot Image
Anti-IVL / Involucrin Antibody (clone SY5) LS-C357940
Mouse Monoclonal [clone SY5] (IgG1,k) to Human IVL / Involucrin
Human, Gorilla, Dog, Pig, Owl monkey
IHC - Paraffin, Immunofluorescence, Flow Cytometry
Unconjugated

100% Guaranteed
Rabbit Polyclonal to Human IVL / Involucrin
Human, Mouse, Rat
IHC - Paraffin, ICC, Immunofluorescence, Western blot
Unconjugated

Details

Human IVL / Involucrin
Rabbit
Human, Mouse, Rat (tested or 100% immunogen sequence identity)
Polyclonal
Unconjugated
Immunoaffinity purified
Unmodified

Applications

  • IHC - Paraffin (1:100 - 1:200)
  • ICC (1:100 - 1:500)
  • Immunofluorescence
  • Western blot (1:500 - 1:1000)

Specificity and Use

KLH-conjugated synthetic peptide encompassing a sequence within the C-terminal region of human Involucrin.
C-Terminus
Recognizes endogenous levels of Involucrin protein.

Packaging

PBS, pH 7.3, 0.01% sodium azide, 30% glycerol.
Store at -20°C. Aliquot to avoid freeze/thaw cycles.
For research use only.

About IVL / Involucrin

P07476 NM_005547 NP_005538.2

IVL Antibody, Involucrin Antibody

Part of the insoluble cornified cell envelope (CE) of stratified squamous epithelia.

Immunohistochemistry

Immunohistochemistry
Immunohistochemical analysis of Involucrin staining in human lung cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.

Immunofluorescence

Immunofluorescence
Immunofluorescent analysis of Involucrin staining in MCF7 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).

Western blot

Western blot
Western blot analysis of Involucrin expression in MCF7 (A); A375 (B); A431 (C); NIH3T3 (D); HT1080 (E); mouse brain (F); rat brain (G) whole cell lysates.

Requested From: 
Date Requested: 11/18/2017

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