Target
Rat ITGAM / CD11b
Synonyms
ITGAM | Antigen CD11b (p170) | CD11B | CD11b antigen | CR3A | Integrin alpha-M | MAC-1 | MO1A | Neutrophil adherence receptor | MAC1A | SLEB6 | CR-3 alpha chain
Reactivity
Rat
(tested or 100% immunogen sequence identity)
Clonality
IgG1
Monoclonal
Conjugations
R. Phycoerythrin
Purification
Ion exchange chromatography
Immunogen
Rat spleen cell homogenate.
Specificity
Recognizes a membrane antigen on rat macrophages, monocytes, dendritic cells and granulocytes. Recognizes small ramified microglia in the central nervous system. No other cell types are positive for ED8. The recognized antigen is a heterodimer (160 and 95kD) belonging to the family of adhesion molecules CD11b/CD18, also designated as Mac-1 antigen or CR3. ED7 and ED8 recognize different epitopes on the same molecule.
Usage
Flow Cytometry: Use 10 ul of the suggested working dilution to label 10^6 cells in 100 ul. Method sheets are available on request. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.
Presentation
PBS, 0.09% Sodium Azide, 5% Sucrose, 1% BSA
Reconstitution
Reconstitute with distilled water.
Storage
Store at 4°C. Do not freeze. Store undiluted. Protect from light. Microcentrifugation recommended if solution contains precipitate.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee