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Anti-ITGA6/Integrin Alpha 6/CD49f Antibody (aa1043-1072) IHC-plus™ LS-B3626

Note: This antibody replaces LS-C98630

Ordering

Wt. Vol. Conc. Price
- 200 µl - $395
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)
206-374-1102
866-206-6909
Orders@LSBio.com
 

Most Popular ITGA6/Integrin Alpha 6/CD49f Antibodies

Anti-ITGA6/Integrin Alpha 6/CD49f Antibody (Internal) IHC-plus™ LS-A8768
Rabbit Polyclonal to Human ITGA6/Integrin Alpha 6/CD49f
Human, Monkey, Mouse, Rat, Bovine, Dog, Guinea pig, Hamster, Horse
IHC - Paraffin
Unconjugated
Immunohistochemistry Image
Anti-ITGA6/Integrin Alpha 6/CD49f Antibody (N-Terminus) IHC-plus™ LS-A8770
Rabbit Polyclonal to Human ITGA6/Integrin Alpha 6/CD49f
Human, Monkey, Mouse, Rat, Bat, Bovine, Dog, Hamster, Horse
IHC - Paraffin
Unconjugated
Immunohistochemistry Image
Anti-ITGA6/Integrin Alpha 6/CD49f Antibody IHC-plus™ LS-B11070
Mouse Monoclonal (IgG1) to Human ITGA6/Integrin Alpha 6/CD49f
Human
IHC - Paraffin, Immunofluorescence, Western blot, Flow Cytometry
Unconjugated
Immunohistochemistry Image

100% Guaranteed 100% Guaranteed
Rabbit Polyclonal to Human ITGA6/Integrin Alpha 6/CD49f
Human
IHC - Paraffin, Immunofluorescence, Western blot
Unconjugated

Details

Human ITGA6/Integrin Alpha 6/CD49f
Rabbit
Human (tested or 100% immunogen sequence identity)
Polyclonal
Unconjugated
Immunoaffinity purified
Unmodified

Applications

  • IHC - Paraffin (5 µg/ml)
  • Immunofluorescence (1:200)
  • Western blot (1:1000)

Specificity and Use

ITGA6/Integrin Alpha 6/CD49f antibody was raised against kLH-conjugated synthetic peptide between amino acids 1043-1072 from human ITGA6.
LS-E7071 - Lyophilized - 100 µg - $145.00
aa1043-1072
Immunohistochemistry: LS-B3626 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B3626 was determined to be 5 ug/ml.

Packaging

PBS, 0.09% sodium azide
Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C.
For research use only.

About ITGA6/Integrin Alpha 6/CD49f

P23229 NM_000210 NP_000201.2

ITGA6 Antibody, CD49f Antibody, CD49f antigen Antibody, Integrin alpha6B Antibody, Integrin, alpha 6 Antibody, Integrin alpha-6 precursor Antibody, VLA-6 Antibody, Integrin alpha-6 Antibody, ITGA6B Antibody

The ITGA6 protein product is the integrin alpha chain alpha 6. Integrins are integral cell-surface proteins composed of an alpha chain and a beta chain. A given chain may combine with multiple partners resulting in different integrins. For example, alpha 6 may combine with beta 4 in the integrin referred to as TSP180, or with beta 1 in the integrin VLA-6. Integrins are known to participate in cell adhesion as well as cell-surface mediated signalling.

Immunohistochemistry

Immunohistochemistry
Anti-ITGA6 / CD49f antibody IHC of human colon. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B3626 concentration 5 ug/ml.

Immunohistochemistry

Immunohistochemistry
Anti-ITGA6 / CD49f antibody IHC of human breast. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B3626 concentration 5 ug/ml.

Immunofluorescence

Immunofluorescence
Fluorescent confocal image of HeLa cells stained with ITGA6 (isoform 2 S1064) antibody. HeLa cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min). Cells were then incubated ITGA6 (isoform 2 S1064) primary antibody (1:200, 2 h at room temperature). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:1000, 1h). Nuclei were counterstained with Hoechst 33342 (blue) (10 ug/ml, 5 min). Note the highly specific localization of the ITGA6 mainly to the nucleus, supported by Human Protein Atlas Data (http://www.proteinatlas.org/ENSG00000091409).

Western blot

Western blot
Western blot of anti-ITGA6 Antibody (isoform 2 S1064) in 293 cell line lysates (35 ug/lane). ITGA6(arrow) was detected using the purified antibody.

Requested From: United States
Date Requested: 12/5/2016

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