Target
Human ITGA4 / VLA-4 / CD49d
Synonyms
ITGA4 | 269C wild type | CD49D | IA4 | Integrin alpha-4 | Integrin alpha 4 | Integrin alpha-4 subunit | Integrin alpha-IV | VLA-4 | VLA-4 subunit alpha | CD49d antigen
Reactivity
Human
(tested or 100% immunogen sequence identity)
Clonality
IgG1
Monoclonal
Conjugations
R. Phycoerythrin
Purification
Ion exchange chromatography
Immunogen
HOON pre-B leukaemia cell line.
Specificity
Recognizes CD49d, also known as integrin alpha 4 subunit, which associates with either integrin beta 1 (to form VLA-4) or beta 7. CD49d is expressed by lymphocytes, monocytes, eosinophils and thymocytes. VLA-4 mediates cell adhesion to VCAM-1 and fibronectin. Clone 44H6 is reported to induce homotypic aggregation. XXX has not confirmed this work.
Usage
Flow Cytometry: Use 10 ul of the suggested working dilution to label 10^6 cells or 100 ul whole blood. Method sheets are available on request. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.
Presentation
PBS, 0.09% Sodium Azide, 5% Sucrose, 1% BSA
Reconstitution
Reconstitute with distilled water.
Storage
Store at 4°C. Do not freeze. Store undiluted. Protect from light. Microcentrifugation recommended if solution contains precipitate.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About ITGA4 / VLA-4 / CD49d