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Catalog Number Size Price
LS-A9385-50 50 µg (1 mg/ml) $440 
ITGA4 / VLA-4 / CD49d Antibody - Anti-VLA-4 / CD49D antibody IHC of human tonsil. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 10-20 ug/ml.
ITGA4 / VLA-4 / CD49d Antibody - Anti-VLA-4 / CD49D antibody IHC of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 10-20 ug/ml.
ITGA4 / VLA-4 / CD49d Antibody - Anti-VLA-4 / CD49D antibody IHC of human tonsil. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 10-20 ug/ml.
ITGA4 / VLA-4 / CD49d Antibody - Anti-VLA-4 / CD49D antibody IHC of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 10-20 ug/ml.
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IHC‑plus™ Polyclonal Rabbit anti‑Human ITGA4 / VLA‑4 / CD49d Antibody (Internal, IHC) LS‑A9385

IHC‑plus™ Polyclonal Rabbit anti‑Human ITGA4 / VLA‑4 / CD49d Antibody (Internal, IHC) LS‑A9385

Antibody:
ITGA4 / VLA-4 / CD49d Rabbit anti-Human Polyclonal (Internal) Antibody
Application:
IHC, IHC-P
Reactivity:
Human, Monkey, Mouse, Rat
Format:
Unconjugated, Unmodified
Other formats:
Price
Catalog Number
$440
LS-A9385-50

Toll Free North America
866-819-4732
For Research Use Only

Overview

Antibody:
ITGA4 / VLA-4 / CD49d Rabbit anti-Human Polyclonal (Internal) Antibody
Application:
IHC, IHC-P
Reactivity:
Human, Monkey, Mouse, Rat
Format:
Unconjugated, Unmodified
Other formats:

Specifications

Description
CD49d antibody LS-A9385 is an unconjugated rabbit polyclonal antibody to CD49d (ITGA4 / VLA-4) (Internal) from human. It is reactive with human, mouse, rat and other species. Validated for IHC. Tested on 20 paraffin-embedded human tissues. Cited in 1 publication.
Target
Human ITGA4 / VLA-4 / CD49d
Synonyms
ITGA4 | 269C wild type | CD49D | IA4 | Integrin alpha-4 | Integrin alpha 4 | Integrin alpha-4 subunit | Integrin alpha-IV | VLA-4 | VLA-4 subunit alpha | CD49d antigen
Host
Rabbit
Reactivity
Human, Monkey, Mouse, Rat (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated. Also available conjugated with AP, APC, Biotin, FITC, HRP, PE.
Purification
Immunoaffinity purified
Modifications
Unmodified
Immunogen
Synthetic 14 amino acid peptide from internal region of human ITGA4 / VLA-4. Percent identity with other species by BLAST analysis: Human (100%), Gorilla (100%), Gibbon (100%), Monkey (100%), Marmoset (100%), Mouse (100%), Rat (100%), Elephant (93%), Panda (93%), Dog (86%), Bat (86%), Bovine (86%), Rabbit (86%), Horse (86%), Pig (86%).
Epitope
Internal
Specificity
Human ITGA4 / VLA-4. BLAST analysis of the peptide immunogen showed no homology with other human proteins.
Applications
  • IHC
  • IHC - Paraffin (10 - 20 µg/ml)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Usage
Immunohistochemistry: The unconjugated form was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for the unconjugated form was determined to be 10 - 20 ug/ml.
Presentation
PBS, 0.1% Sodium Azide
Storage
Aliquot and store undiluted at -20°C or below for up to 1 year. Can be stored undiluted at 4°C for up to 1 month. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About ITGA4 / VLA-4 / CD49d
P13612 NM_000885 NP_000876.3

Validation

ITGA4 / VLA-4 / CD49d Antibody - Anti-VLA-4 / CD49D antibody IHC of human tonsil. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 10-20 ug/ml.
Anti-VLA-4 / CD49D antibody IHC of human tonsil. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 10-20 ug/ml.
Anti-VLA-4 / CD49D antibody IHC of human tonsil. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 10-20 ug/ml.

ITGA4 / VLA-4 / CD49d Antibody - Anti-VLA-4 / CD49D antibody IHC of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 10-20 ug/ml.
Anti-VLA-4 / CD49D antibody IHC of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 10-20 ug/ml.
Anti-VLA-4 / CD49D antibody IHC of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 10-20 ug/ml.

LSBio Ratings

IHC-plus™ ITGA4 / VLA-4 / CD49d Antibody (Internal) for IHC LS-A9385 has an LSBio Rating of
Publications (4)
Laboratory Validation Score (4)

Publications (1)

Clinical relevance of integrin alpha 4 in gastrointestinal stromal tumours. Pulkka OP, Mpindi JP, Tynninen O, Nilsson B, Kallioniemi O, Sihto H, Joensuu H. Journal of cellular and molecular medicine. 2018 April;22:2220-2230. (Human)

Customer Reviews (0)


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Requested From: United States
Date Requested: 5/25/2022