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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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ITCH / AIP4 Antibody (aa386‑435) LS‑C118343 Best Seller
AIP4 antibody LS-C118343 is an unconjugated rabbit polyclonal antibody to AIP4 (ITCH) from human and mouse. Validated for Peptide-ELISA and WB.
Catalog
Size
Price
LS-C118343-50
50 µl
Unavailable
LS-C118343-100
100 µl
Unavailable

Popular ITCH / AIP4 Products

Detection of Human Itch by Western Blot. Samples: Whole cell lysate (5, 15 and 50 ug) from Jurkat cells. Antibodies: Affinity purified rabbit anti-Itch antibody used for WB at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.
Species: Human, Mouse, Rat, Bovine, Dog, Chicken, Xenopus
Applications: Western blot
Antibody
Species: Human
Applications: Immunofluorescence, ELISA
Antibody
Species: Human
Applications: Flow Cytometry
Human Liver: Formalin-Fixed, Paraffin-Embedded (FFPE)
Species: Human
Applications: IHC, IHC - Paraffin, ICC, Immunofluorescence, Flow Cytometry

Product Description

AIP4 antibody LS-C118343 is an unconjugated rabbit polyclonal antibody to AIP4 (ITCH) from human and mouse. Validated for Peptide-ELISA and WB.
About ITCH / AIP4
Acts as an E3 ubiquitin-protein ligase which accepts ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates. It catalyzes 'Lys-29'-, 'Lys-48'- and 'Lys-63'-linked ubiquitin conjugation. It is involved in the control of inflammatory signaling pathways. Q96J02 NM_031483 NP_113671.3

ITCH Antibody, AIF4 Antibody, AIP4 Antibody, DJ468O1.1 Antibody, NAPP1 Antibody, NFE2-associated polypeptide 1 Antibody


Specifications

Target
Human ITCH / AIP4
Host
Rabbit
Reactivity
Human, Mouse (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated
Purification
Immunoaffinity purified
Modifications
Unmodified
Applications
  • Western blot (1:500 - 1:1000)
  • Peptide Enzyme-Linked Immunosorbent Assay (1:40000)
Failed Applications
  • IHC - Paraffin
Immunogen
ITCH / AIP4 antibody was raised against synthetic peptide from human ITCH (aa386-435).
Epitope
aa386-435
Specificity
ITCH (Ab-420) Antibody detects endogenous levels of total ITCH protein.
Presentation
PBS (without Mg2+, Ca2+), pH 7.4, 150 mM sodium chloride, 0.02% sodium azide, 50% glycerol
Storage
Store at -20°C for up to 1 year.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.

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Images


Western blot

Western blot of extracts from mouse brain cells, using ITCH (Ab-420) Antibody. The lane on the right is treated with the synthesized peptide.
Western blot of extracts from mouse brain cells, using ITCH (Ab-420) Antibody. The lane on the right is treated with the synthesized peptide.

Western blot

Western blot of extracts from mouse brain cells, using ITCH (Ab-420) Antibody. The lane on the right is treated with the synthesized peptide.
Western blot of extracts from mouse brain cells, using ITCH (Ab-420) Antibody. The lane on the right is treated with the synthesized peptide.

Western blot

Western blot of extracts from mouse brain cells, using ITCH (Ab-420) Antibody. The lane on the right is treated with the synthesized peptide.
Western blot of extracts from mouse brain cells, using ITCH (Ab-420) Antibody. The lane on the right is treated with the synthesized peptide.

Western blot

Western blot of extracts from mouse brain cells, using ITCH (Ab-420) Antibody. The lane on the right is treated with the synthesized peptide.
Western blot of extracts from mouse brain cells, using ITCH (Ab-420) Antibody. The lane on the right is treated with the synthesized peptide.


Requested From: 
Date Requested: 6/18/2018

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