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Anti-IRF3 Antibody (aa206-427) LS-C36964

Catalog Size Price
LS-C36964-100 100 µg (1 mg/ml) Unavailable

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112 IRF3 Antibodies

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100% Guaranteed
Goat Polyclonal (IgG) to Human IRF3
Human
ICC, Western blot, Flow Cytometry, ELISA
Unconjugated

Details

Human IRF3
Goat
Human (tested or 100% immunogen sequence identity)
IgG Polyclonal
Unconjugated
Affinity purified
Unmodified

Applications

  • ICC (10 µg/ml)
  • Western blot (1 µg/ml)
  • Flow Cytometry (50 µg/ml)
  • ELISA (0.5 - 1 µg/ml)

Specificity and Use

IRF3 antibody was raised against e. coli-derived rhIRF3 (aa206-427).
aa206-427
Selected for its ability to recognize human IRF3 in the applications listed below. In direct ELISA and Western Blots, this antibody shows ~60% cross-reactivity with rmIRF3.
Suitable for use in Western Blot, Flow Cytometry, Immunocytochemistry and ELISA. Western Blot: 1 ug/ml (w/ appropriate secondary reagents). Detection limit is ~10 ng/lane (non-reducing) and ~1 ng/lane (reducing). Flow Cytometry: 50 ug/ml and add 10 ul of the diluted solution to 1-2.5x10^5 HeLa cells in a total reaction volume 200ul. The binding of unlabeled antibodies may be visualized by adding 10 ul of a 25 ug/ml stock solution of a secondary developing reagent such as anti-goat IgG (FITC). Immunocytochemistry: 10 ug/ml detects human IRF3 in HeLa cells. Cells were fixed with PBS, 4% paraformaldehyde for 20 min at RT and blocked with PBS, 10% normal donkey serum, 0.1% Triton X-100, and 1% BSA for 45 min at RT. After blocking, cells were incubated with diluted primary antibody overnight at 4? C followed by fluorochrome-coupled anti-goat IgG at RT in the dark for one hour. Between each step, cells were washed with PBS, 0.1% BSA. ELISA: 0.5-1.0 ug/ml (w/ appropriate secondary reagents), detection limit is ~0.2 ng/well.

Packaging

Lyophilized.
100 µl 40-50% glycerol, PBS.
+4°C or -20°C, Avoid repeated freezing and thawing.
For research use only.

About IRF3

Q14653 NM_001571 NP_001562.1

IRF3 Antibody, Interferon regulatory factor 3 Antibody, IRF-3 Antibody

Key transcriptional regulator of type I interferon (IFN)-dependent immune responses which plays a critical role in the innate immune response against DNA and RNA viruses. Regulates the transcription of type I IFN genes (IFN-alpha and IFN-beta) and IFN-stimulated genes (ISG) by binding to an interferon-stimulated response element (ISRE) in their promoters.

Requested From: 
Date Requested: 8/21/2017

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