Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to
worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting
immunostaining in relation to complex human pathologies.
TCR Screening Services
Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding.
Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".
IHC - Paraffin, ICC, Western blot, Immunoprecipitation
Human IPO13 / LGL2
Human, Mouse, Rat, Bovine (tested or 100% immunogen sequence identity)
IHC - Paraffin (1:100 - 1:1000)
ICC (1:100 - 1:1000)
Western blot (1:500 - 1:3000)
Specificity and Use
IPO13 / LGL2 antibody was raised against synthetic peptide corresponding to a region within amino acids 901 and 963 of Importin 13 (SwissProt O94829). Percent identity by BLAST analysis: Human, Mouse, Rat, Bovine (100%).
Human IPO13 / LGL2
IHC-paraffin: Suggested antigen retrieval using heat mediated 10 mM Citrate buffer (pH 6.0) or Tris-EDTA buffer (pH 8.0).
0.1 M Tris-glycine, pH 7.0, 10% glycerol, 0.01% Thimerosal
Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
IPO13 Antibody, IMP13 Antibody, Importin-13 Antibody, Karyopherin 13 Antibody, Late gestation lung 2 Antibody, KIAA0724 Antibody, Ran binding protein 13 Antibody, Ran-binding protein 13 Antibody, Importin 13 Antibody, KAP13 Antibody, Karyopherin-13 Antibody, LGL2 Antibody, RANBP13 Antibody
Functions in nuclear protein import as nuclear transport receptor. Serves as receptor for nuclear localization signals (NLS) in cargo substrates. Is thought to mediate docking of the importin/substrate complex to the nuclear pore complex (NPC) through binding to nucleoporin and the complex is subsequently translocated through the pore by an energy requiring, Ran-dependent mechanism.
IHC of paraffin-embedded CL1-5 xenograft using Importin 13 antibody at 1:100 dilution.
Immunofluorescence of paraformaldehyde-fixed HeLa using Importin 13 antibody at 1:200 dilution.
Western blot of IPO13 expression in transfected 293T cell line by IPO13 polyclonal antibody. A: Non-transfected lysate., B: IPO13 transfected lysate. 7.5% SDS PAGE. IPO13 / LGL2 antibody diluted at 1:500
IPO13 was immunoprecipitated from 1 mg of HeLa whole cell lysate using 10 ug of anti-IPO13 antibody . The precipitated IPO13 was detected by IPO13 / LGL2 antibody diluted at 1:1000.
Requested From: United States
Date Requested: 10/25/2016