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Anti-Insulin Antibody IHC-plus™ LS-B3297

Note: This antibody replaces LS-C24670

Ordering

Wt. Vol. Conc. Price
50 µg - 1 mg/ml $395
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)
206-374-1102
866-206-6909
Orders@LSBio.com
 

Most Popular Insulin Antibodies

Anti-Insulin Antibody (clone 8E2, HRP) IHC-plus™ LS-B2407
Mouse Monoclonal [clone 8E2] (IgG1,k) to Human Insulin
Human
IHC - Paraffin, ELISA
HRP Conjugated
Immunohistochemistry Image
Anti-Insulin Antibody IHC-plus™ LS-B2526
Rabbit Polyclonal (IgG) to Human Insulin
Human, Mouse, Rat
IHC - Paraffin, ICC, Immunofluorescence, Flow Cytometry
Unconjugated
Immunohistochemistry Image
Anti-Insulin Antibody LS-C190934
Guinea pig Polyclonal (IgG) to Human Insulin
Human, Bovine, Pig
ELISA
Unconjugated

100% Guaranteed 100% Guaranteed
Mouse Monoclonal (IgG1) to Human Insulin
Human
IHC - Paraffin, ELISA
Unconjugated

Details

Human Insulin
Mouse
Human (tested or 100% immunogen sequence identity)
IgG1 Monoclonal
Unconjugated
Purified
Unmodified

Applications

  • IHC - Paraffin (10 µg/ml)
  • ELISA

Specificity and Use

Insulin antibody was raised against human insulin.
Recognizes Insulin: 100%, Pro-Insulin: 0.2 %. The epitope has been determined and it maps to a C-terminal pentapeptide of human insulin beta-chain.
Immunohistochemistry: LS-B3297 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B3297 was determined to be 10 ug/ml.

Packaging

PBS, 0.1% sodium azide, pH 7.4.
Long term: -20°C; Short term: +4°C; Avoid freeze-thaw cycles.
For research use only.

About Insulin

P01308 NM_000207 NP_000198.1

INS Antibody, IRDN Antibody, IDDM2 Antibody, Preproinsulin Antibody, ILPR Antibody, Insulin Antibody, MODY10 Antibody, Proinsulin Antibody

After removal of the precursor signal peptide, proinsulin is post-translationally cleaved into three peptides: the B chain and A chain peptides, which are covalently linked via two disulfide bonds to form insulin, and C-peptide. Binding of insulin to the insulin receptor (INSR) stimulates glucose uptake. A multitude of mutant alleles with phenotypic effects have been identified.

Immunohistochemistry

Immunohistochemistry
Anti-Insulin antibody IHC of human pancreas. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B3297 concentration 10 ug/ml.
 

Requested From: United States
Date Requested: 12/2/2016

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