Pig Insulin

Guinea pig

Pig (tested or 100% immunogen sequence identity)

Polyclonal

Unconjugated. Also available conjugated with FITC.

Purified IgG

Unmodified

Insulin is a hormone produced by the beta cells of the islets of Langerhans in the pancreas. It plays an important role in the metabolism of carbohydrate by its effects on the storage of glycogen in the liver, the conversion of carbohydrate to fat, and the oxidation of glucose by the peripheral tissues. Insulin has a molecular weight of 6,000 and contains two peptide chains linked by two disulphide bonds. The hormone is isolated from porcine pancreas. Freund's complete adjuvant is used in the fi

Swine insulin

• Immunofluorescence
• ELISA
• Dot Blot

The reactivity of the antiserum is directed to insulin as tested by ELISA and immunoblotting. No reaction was obtained with other swine serum proteins. The cytochemical grade allows the use in different types of highly sensitive immunoassays on appropriately treated cell and tissue substrates. in radioimmunoassay. for the production of immunoconjugates with a selected marker. to prepare immunoaffinity adsorbents by coupling to an artificial carrier. in non-isotopic methodology based on solid phase immunochemistry (e. g. ELISA), both as catching antibody and detection reagent. in Western blotting. This product is not pre-diluted. The optimum working dilution of each product should be established by titration before being used. Working dilutions for histochemical and cytochemical use are usually between 1:100 and 1:250. in ELISA and comparable non-precipitating antibody-binding assays are between 1:500 and 1:5000.

Lyophilized from PBS, pH 7.2, No preservatives added

Reconstitute with distilled water.

Short term: store at 4°C. Long term: store at -20°C. Avoid freeze-thaw cycles.

For research use only. Intended for use by laboratory professionals.

This antibody carries the LSBio 100% Guarantee.

Uniprot: P01308 NCBI: NM_000207 NP_000198.1