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Anti-IL6 / Interleukin 6 Antibody LS-C154128

Ordering

Wt. Vol. Conc. Price
100 µg 100 µl 1 mg/ml $405
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)
206-374-1102
866-206-6909
Orders@LSBio.com
 

Most Popular IL6 / Interleukin 6 Antibodies

Anti-IL6 / Interleukin 6 Antibody (clone 6D9A1,6C12B1) IHC-plus™ LS-B1582
Mouse Monoclonal [clone 6D9A1,6C12B1] (IgG1) to Human IL6 / Interleukin 6
Human
IHC - Paraffin, Western blot, ELISA
Unconjugated
Immunohistochemistry Image
Anti-IL6 / Interleukin 6 Antibody (clone 10E5) IHC-plus™ LS-B5763
Mouse Monoclonal [clone 10E5] (IgG2b) to Rat IL6 / Interleukin 6
Rat
IHC - Paraffin, Western blot, Flow Cytometry
Unconjugated
Immunohistochemistry Image
Anti-IL6 / Interleukin 6 Antibody IHC-plus™ LS-B7482
Rabbit Polyclonal (IgG) to Human IL6 / Interleukin 6
Human
IHC - Paraffin, Western blot, ELISA, Neutralization
Unconjugated
Immunohistochemistry Image

100% Guaranteed 100% Guaranteed
Rabbit Polyclonal (IgG) to Mouse IL6 / Interleukin 6
Mouse
IHC, Western blot, ELISA
Unconjugated

Details

Mouse IL6 / Interleukin 6
Rabbit
Mouse (tested or 100% immunogen sequence identity)
IgG Polyclonal
Unconjugated
Ion exchange chromatography
Unmodified

Applications

  • IHC
  • Western blot (1:1000)
  • ELISA

Specificity and Use

IL6 / Interleukin 6 antibody was raised against anti-IL-6 is an IgG fraction antibody prepared from rabbit antiserum after repeated immunizations with recombinant mouse IL-6 protein produced in E. coli.
This antibody is specific for mouse IL-6 protein. A BLAST analysis was used to suggest cross-reactivity with IL-6 from mouse sources based on 100% homology with the immunizing sequence. Based on 94% homology, there is a chance of cross-reactivity to IL-6 from rat. Cross-reactivity with IL-6 from other sources has not been determined.
This purified antibody has been tested for use in ELISA and western blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 21.7 kD in size corresponding to the mature 187 amino acid mouse IL-6 protein by western blotting in appropriate cell lysate or extract.

Packaging

0.02 M potassium phosphate, 0.15 M sodium chloride, pH 7.2
100 µl deionized water
Store lyophilized at 4°C. Once reconstituted, aliquot and store at -20°C. Avoid freeze/thaw cycles. Store undiluted.
For research use only.

About IL6 / Interleukin 6

P05231 NM_000600 NP_000591.1

IL6 Antibody, B-cell stimulatory factor 2 Antibody, BSF-2 Antibody, BSF2 Antibody, CTL differentiation factor Antibody, Hybridoma growth factor Antibody, IFN-beta-2 Antibody, Interleukin-6 Antibody, Interferon beta-2 Antibody, Interleukin BSF-2 Antibody, IFNB2 Antibody, B-cell differentiation factor Antibody, CDF Antibody, HSF Antibody, IL-6 Antibody

Cytokine with a wide variety of biological functions. It is a potent inducer of the acute phase response. Plays an essential role in the final differentiation of B-cells into Ig-secreting cells Involved in lymphocyte and monocyte differentiation. Acts on B-cells, T-cells, hepatocytes, hematopoietic progenitor cells and cells of the CNS. Required for the generation of T(H)17 cells. Also acts as a myokine.

Western blot

Western blot
Anti-Mouse IL-6 Antibody - Western Blot. Anti-mouse IL-6 antibody in western blot shows detection of recombinant mouse IL-6 raised in E. coli. Recombinant truncated protein (0.1 ug, 21.7 kD) was loaded on to an SDS-PAGE gel, and after separation, transferred to nitrocellulose. The membrane was blocked with 1% BSA in TBST for 30 min at RT, followed by incubation with Anti-Mouse IL-6 antibody diluted 1:1000 in 1% BSA in TBST overnight at 4°C. After washes, the blot was reacted with secondary antibody Dylight 649 Conjugated Anti-Rabbit IgG (H&L) (Goat) Antibody ( diluted 1:20000 in blocking buffer (MB-070) for 30 min. at RT. Data was collected using Bio-Rad VersaDoc 4000 MP imaging system.
 

Requested From: United States
Date Requested: 12/6/2016

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