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Anti-IL4 Antibody LS-C154091


Wt. Vol. Conc. Price
100 µg 100 µl - $405
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)

Most Popular IL4 Antibodies

Anti-IL4 Antibody (clone 860A 4B3) LS-C7845
Mouse Monoclonal [clone 860A 4B3] (IgG1,k) to Human IL4
Immunoprecipitation, Flow Cytometry, ELISA, Sandwich ELISA
Anti-IL4 Antibody IHC-plus™ LS-B7474
Rabbit Polyclonal (IgG) to Human IL4
IHC - Paraffin, Western blot, ELISA, Neutralization, Sandwich ELISA
Immunohistochemistry Image

100% Guaranteed 100% Guaranteed
Rabbit Polyclonal (IgG) to Human IL4
Western blot, ELISA


Human IL4
Human (tested or 100% immunogen sequence identity)
IgG Polyclonal
Ion exchange chromatography


  • Western blot (1:1000)

Specificity and Use

IL4 antibody was raised against this purified antibody was prepared from whole rabbit serum produced by repeated immunizations with full length recombinant human IL-4 protein.
This purified antibody has been heated to 56°C for 30 minutes. In ELISA and other immunoreactive assays, this antibody will recognize both native and recombinant human IL-4 in cell supernatants and certain body fluids. A control of similarly diluted normal rabbit IgG is recommended.
This purified antibody has been tested for use in ELISA and western blotting. Reactivity is also expected in neutralizations, radioimmunoassay and immunohistochemistry. The endotoxin content is estimated to be <10 pg/ul by the LAL method. By western blot a band approximately 15 kD in size corresponding to native human IL-4 protein is expected in the appropriate cell lysate or extract. Specific conditions for reactivity should be optimized by the end user.


0.02 M potassium phosphate, 0.15 M sodium chloride, pH 7.2
100 µl deionized water
Short term 4°C, long term aliquot and store at -20°C, avoid freeze thaw cycles.
For research use only.

About IL4

P05112 NM_000589 NP_000580.1

IL4 Antibody, B cell growth factor 1 Antibody, B-cell stimulatory factor 1 Antibody, B_cell stimulatory factor 1 Antibody, BCGF1 Antibody, BSF-1 Antibody, Binetrakin Antibody, BCGF-1 Antibody, Interleukin 4 Antibody, Interleukin-4 Antibody, Pitrakinra Antibody, BSF1 Antibody, IL-4 Antibody

IL4 is a pleiotropic cytokine produced by activated T cells. This cytokine is a ligand for interleukin 4 receptor. The interleukin 4 receptor also binds to IL13, which may contribute to many overlapping functions of this cytokine and IL13. STAT6, a signal transducer and activator of transcription, has been shown to play a central role in mediating the immune regulatory signal of this cytokine.

Western blot

Western blot
Anti-IL-4 Antibody - Western Blot. anti-Human IL-4 antibody shows detection of a band ~15 kD in size corresponding to recombinant human IL-4. The identity of the faint higher molecular weight band may represent a homodimer. Molecular weight markers are also shown (left). After transfer, the membrane was blocked overnight with 3% BSA in TBS followed by reaction with primary antibody at a 1:1000 dilution. Detection occurred using peroxidase conjugated anti-Rabbit IgG (LS-C60865) secondary antibody diluted 1:40000 in blocking buffer (p/n MB-070) for 30 min at RT followed by reaction with FemtoMax chemiluminescent substrate. Image was captured using VersaDoc MP 4000 imaging system (Bio-Rad).

Requested From: United States
Date Requested: 10/22/2016

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