Target
Mouse IL4
Synonyms
IL4 | B cell growth factor 1 | B-cell stimulatory factor 1 | B_cell stimulatory factor 1 | BCGF1 | BSF-1 | Binetrakin | BCGF-1 | Interleukin 4 | Interleukin-4 | Pitrakinra | BSF1 | IL-4
Reactivity
Mouse
(tested or 100% immunogen sequence identity)
Clonality
IgG2b,k
Monoclonal
Conjugations
Unconjugated
Immunogen
E. coli-expressed recombinant mouse IL-4.
Specificity
Recognizes mouse Interleukin-4 (IL-4).
Applications
- Immunofluorescence
- Western blot
- Flow Cytometry
- ELISA
- Neutralization
Usage
ELISA Capture: Useful as a capture antibody in a sandwich ELISA for quantifying mouse IL-4 protein levels.1-4 Purified antibody should be paired with biotinylated antibody (Cat. Immunofluorescence/. Flow Cytometry: Useful for intracytoplasmic staining and flow cytometric analysis to identify and enumerate IL-4-positive cells within mixed cell populations. Recombinant mouse IL-4, unlabeled Rat IgG2b should be used as a specificity control. Western Blot: Useful for Western blot applications. For Western blotting, a concentration of 1-5 ug/ml (in conjunction with AP-labeled Goat Anti-Rat-Ig) has been found to enable detection of 100 ng/lane of recombinant mouse IL-4 under reducing conditions. Neutralization: The LE/AF form can be used for neutralizing mouse IL-4 bioactivity. A suitable LE/AF Rat IgG2b should be used as an isotype control. The applications listed have been tested for the unmodified form of this product. Other forms have not been tested.
Presentation
PBS, No preservatives added
Storage
Short term: 4°C; Long term: Add glycerol (40-50%) -20°C.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee