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Anti-IL36RN / IL1F5 Antibody LS-C154077

Catalog Size Price
LS-C154077-100 100 µg Unavailable

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100% Guaranteed
Rabbit Polyclonal (IgG) to Bovine IL36RN / IL1F5
Western blot, ELISA


Bovine IL36RN / IL1F5
Bovine (tested or 100% immunogen sequence identity)
IgG Polyclonal
Protein A purified


  • Western blot (1 µg/ml)

Specificity and Use

IL36RN / IL1F5 antibody was raised against recombinant protein produced in yeast, corresponding to the 155 amino acids of the mature bovine IL-1F5 protein.
This antibody is specific for bovine IL-1F5 protein. A BLAST analysis was used to suggest cross-reactivity with IL-1F5 from dog and mouse sources based on 94% homology with the immunizing sequence. Partial reactivity is expected against horse based on 91% homology, and to human, chimpanzee, macaque, and rat based on 90% homology with the immunizing sequence.
This protein-A purified antibody is suitable for ELISA and Western blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 17.1 kD in size corresponding to bovine IL-1F5 protein by western blotting in the appropriate cell lysate or extract.


0.02 M potassium phosphate, 0.15 M sodium chloride, pH 7.2, 0.01% sodium azide
100 µl deionized water
Short term 4°C, long term aliquot and store at -20°C, avoid freeze thaw cycles.
For research use only.

About IL36RN / IL1F5

Q9UBH0 NM_012275 NP_036407.1

IL36RN Antibody, FIL1 Antibody, FIL1D Antibody, IL-1 delta Antibody, IL-1 related protein 3 Antibody, IL-1HY1 Antibody, IL-1RP3 Antibody, IL-1-related protein 3 Antibody, IL1F5 Antibody, IL1RP3 Antibody, IL1L1 Antibody, IL36RA Antibody, Interleukin-1 delta Antibody, Family of interleukin 1-delta Antibody, FIL1 delta Antibody, IL-1L1 Antibody, IL-1ra homolog 1 Antibody, Interleukin-1 family member 5 Antibody, Interleukin-1-like protein 1 Antibody, FIL1(DELTA) Antibody, IL-1F5 Antibody, IL1HY1 Antibody, Interleukin-1 HY1 Antibody, PSORP Antibody

Inhibits the activity of interleukin-36 (IL36A,IL36B and IL36G) by binding to receptor IL1RL2 and preventing its association with the coreceptor IL1RAP for signaling. Part of the IL-36 signaling system that is thought to be present in epithelial barriers and to take part in local inflammatory response; similar to the IL-1 system with which it shares the coreceptor. Proposed to play a role in skin inflammation.

Western blot

Western blot
Anti-Bovine IL-1F5 Antibody - Western Blot. Western blot of Protein-A Purified anti-bovine IL-1F5 antibody shows detection of recombinant bovine IL-1F5 at 17.1 kD (arrowhead) raised in yeast. The identity of the band at 36 kD is not known. The protein was purified and resolved by SDS-PAGE, then transferred to PVDF membrane. Membrane was blocked with 3% BSA (BSA-30, diluted 1:10), and probed with 1 ug/mL primary antibody overnight at 4C. After washing, membrane was probed with IRDye800 Conjugated Goat Anti-Rabbit IgG ( for 45 min at room temperature.

Requested From: 
Date Requested: 3/20/2018

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