Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to
worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting
immunostaining in relation to complex human pathologies.
TCR Screening Services
Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding.
Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".
Human (tested or 100% immunogen sequence identity)
Western blot (1:250 - 1:2500)
ELISA (1:500 - 1:10000)
Specificity and Use
IL12B / IL12 p40 antibody was raised against recombinant human IL-23p40: Fc (interleukin-23p40: Fc).
Recognizes human IL-23p40. Does not cross-react with human IL-23p19.
Suitable for use in ELISA and Western Blot. ELISA (direct and indirect): 1:250-1:5000. Western Blot: 1:250-1:2500 using ECL; Suggested blocking and dilution buffer is TBST, 0.05% Tween 20 and 5% skim milk; suggested incubation time is 1 hour at RT.
PBS, pH 7.4, 40% glycerol. No preservatives.
Long term: -20°C; Short term: +4°C. Avoid repeat freeze-thaw cycles.
IL12B / IL12 p40 is a subunit of interleukin 12, a cytokine that acts on T and natural killer cells, and has a broad array of biological activities. Interleukin 12 is a disulfide-linked heterodimer composed of the 40 kD cytokine receptor like subunit encoded by this gene, and a 35 kD subunit encoded by IL12A. This cytokine is expressed by activated macrophages that serve as an essential inducer of Th1 cells development.