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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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IL12B / IL12 p40 Antibody (aa264‑290) LS‑C165172
IL12 p40 antibody LS-C165172 is an unconjugated rabbit polyclonal antibody to human IL12 p40 (IL12B). Validated for Flow, IHC and WB.
Catalog
Size
Price
LS-C165172-400
400 µl
$305

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Product Description

IL12 p40 antibody LS-C165172 is an unconjugated rabbit polyclonal antibody to human IL12 p40 (IL12B). Validated for Flow, IHC and WB.
About IL12B / IL12 p40
IL12B / IL12 p40 is a subunit of interleukin 12, a cytokine that acts on T and natural killer cells, and has a broad array of biological activities. Interleukin 12 is a disulfide-linked heterodimer composed of the 40 kD cytokine receptor like subunit encoded by this gene, and a 35 kD subunit encoded by IL12A. This cytokine is expressed by activated macrophages that serve as an essential inducer of Th1 cells development. P29460 NM_002187 NP_002178.2

IL12B Antibody, CLMF p40 Antibody, IL-12 p40 Antibody, IL-12 subunit p40 Antibody, Interleukin-12 beta chain Antibody, Interleukin-12 subunit beta Antibody, IL12, subunit p40 Antibody, Interleukinl-12 Antibody, Interleukin 12, p40 Antibody, NKSF Antibody, CLMF Antibody, CLMF2 Antibody, IL-12B Antibody, NKSF2 Antibody


Specifications

Target
Human IL12B / IL12 p40
Host
Rabbit
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Protein A purified
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin (1:50 - 1:100)
  • Western blot (1:1000)
  • Flow Cytometry (1:10 - 1:50)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Blocking Peptide
LS-E227 - Lyophilized - 100 µg - $145.00
Immunizing peptide used to generate LS-C165172. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
Epitope
aa264-290
Specificity
This IL12B antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 264-290 amino acids from the C-terminal region of human IL12B.
Presentation
PBS, 0.09% Sodium Azide
Storage
Maintain refrigerated at 2°C to 8°C for up to 6 months. For long term storage store at -20°C.
Restrictions
For research use only.

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Images

Immunohistochemistry

IL12B antibody immunohistochemistry of formalin-fixed and paraffin-embedded human tonsil tissue followed by peroxidase-conjugated secondary antibody and DAB staining.
IL12B antibody immunohistochemistry of formalin-fixed and paraffin-embedded human tonsil tissue followed by peroxidase-conjugated secondary antibody and DAB staining.

Western blot

IL12B Antibody western blot of CEM,MDA-MB453 cell line lysates (35 ug/lane). The IL12B antibody detected the IL12B protein (arrow).
IL12B Antibody western blot of CEM,MDA-MB453 cell line lysates (35 ug/lane). The IL12B antibody detected the IL12B protein (arrow).

Flow Cytometry

IL12B Antibody flow cytometry of MDA-MB435 cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
IL12B Antibody flow cytometry of MDA-MB435 cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Immunohistochemistry

IL12B antibody immunohistochemistry of formalin-fixed and paraffin-embedded human tonsil tissue followed by peroxidase-conjugated secondary antibody and DAB staining.
IL12B antibody immunohistochemistry of formalin-fixed and paraffin-embedded human tonsil tissue followed by peroxidase-conjugated secondary antibody and DAB staining.

Western blot

IL12B Antibody western blot of CEM,MDA-MB453 cell line lysates (35 ug/lane). The IL12B antibody detected the IL12B protein (arrow).
IL12B Antibody western blot of CEM,MDA-MB453 cell line lysates (35 ug/lane). The IL12B antibody detected the IL12B protein (arrow).

Flow Cytometry

IL12B Antibody flow cytometry of MDA-MB435 cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
IL12B Antibody flow cytometry of MDA-MB435 cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Immunohistochemistry

IL12B antibody immunohistochemistry of formalin-fixed and paraffin-embedded human tonsil tissue followed by peroxidase-conjugated secondary antibody and DAB staining.
IL12B antibody immunohistochemistry of formalin-fixed and paraffin-embedded human tonsil tissue followed by peroxidase-conjugated secondary antibody and DAB staining.

Western blot

IL12B Antibody western blot of CEM,MDA-MB453 cell line lysates (35 ug/lane). The IL12B antibody detected the IL12B protein (arrow).
IL12B Antibody western blot of CEM,MDA-MB453 cell line lysates (35 ug/lane). The IL12B antibody detected the IL12B protein (arrow).

Flow Cytometry

IL12B Antibody flow cytometry of MDA-MB435 cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
IL12B Antibody flow cytometry of MDA-MB435 cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Immunohistochemistry

IL12B antibody immunohistochemistry of formalin-fixed and paraffin-embedded human tonsil tissue followed by peroxidase-conjugated secondary antibody and DAB staining.
IL12B antibody immunohistochemistry of formalin-fixed and paraffin-embedded human tonsil tissue followed by peroxidase-conjugated secondary antibody and DAB staining.

Western blot

IL12B Antibody western blot of CEM,MDA-MB453 cell line lysates (35 ug/lane). The IL12B antibody detected the IL12B protein (arrow).
IL12B Antibody western blot of CEM,MDA-MB453 cell line lysates (35 ug/lane). The IL12B antibody detected the IL12B protein (arrow).

Flow Cytometry

IL12B Antibody flow cytometry of MDA-MB435 cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
IL12B Antibody flow cytometry of MDA-MB435 cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Requested From: United States
Date Requested: 9/22/2018

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