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Anti-IGF2 Antibody (clone 8H1) LS-C169113

Catalog Size Price
LS-C169113-100 100 µg (1 mg/ml) Unavailable

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100% Guaranteed
Mouse Monoclonal [clone 8H1] (IgG1) to Human IGF2
Human
IHC, IHC - Paraffin, Immunofluorescence, Western blot, Flow Cytometry, ELISA
Unconjugated

Details

Human IGF2
Mouse
Human (tested or 100% immunogen sequence identity)
IgG1 Monoclonal [8H1]
Unconjugated
Purified
Unmodified

Applications

  • IHC
  • IHC - Paraffin (1:200 - 1:1000)
  • Immunofluorescence (1:200 - 1:1000)
  • Western blot (1:100 - 1:500)
  • Flow Cytometry (1:200 - 1:400)
  • ELISA (1:10000)

Specificity and Use

IGF2 antibody was raised against purified recombinant fragment of human IGF2 (AA: 25-180) expressed in E. Coli.
Human

Packaging

Purified antibody in PBS containing 0.05% sodium azide
Short term: 4°C; Long term: -20°C.
For research use only.

About IGF2

P01344 NM_000612 NP_000603.1

IGF2 Antibody, C11orf43 Antibody, Insulin-like growth factor II Antibody, IGF-II Antibody, PP9974 Antibody, Somatomedin-A Antibody

The insulin-like growth factors possess growth-promoting activity. In vitro, they are potent mitogens for cultured cells. IGF-II is influenced by placental lactogen and may play a role in fetal development.Preptin undergoes glucose-mediated co-secretion with insulin, and acts as physiological amplifier of glucose-mediated insulin secretion. Exhibits osteogenic properties by increasing osteoblast mitogenic activity through phosphoactivation of MAPK1 and MAPK3.

Immunohistochemistry

IHC of paraffin-embedded ovarian cancer tissues using IGF2 mouse monoclonal antibody with DAB staining.
IHC of paraffin-embedded ovarian cancer tissues using IGF2 mouse monoclonal antibody with DAB staining.

Immunohistochemistry

IHC of paraffin-embedded bladder cancer tissues using IGF2 mouse monoclonal antibody with DAB staining.
IHC of paraffin-embedded bladder cancer tissues using IGF2 mouse monoclonal antibody with DAB staining.

Immunofluorescence

Immunofluorescence of HeLa cells using IGF2 mouse monoclonal antibody (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
Immunofluorescence of HeLa cells using IGF2 mouse monoclonal antibody (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.

Western blot

Western blot using IGF2 monoclonal antibody against human IGF2 recombinant protein. (Expected MW is 43.1 kDa)
Western blot using IGF2 monoclonal antibody against human IGF2 recombinant protein. (Expected MW is 43.1 kDa)

Western blot

Western blot using IGF2 monoclonal antibody against HEK293 (1) and IGF2 (AA: 25-180)-hIgGFc transfected HEK293 (2) cell lysate.
Western blot using IGF2 monoclonal antibody against HEK293 (1) and IGF2 (AA: 25-180)-hIgGFc transfected HEK293 (2) cell lysate.

Enzyme-Linked Immunosorbent Assay

Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);
Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);

Requested From: 
Date Requested: 5/23/2018

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