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Anti-IGF-II / IGF2 Antibody IHC-plus™ LS-B7415

Note: This antibody replaces LS-C104357, LS-C42967

Ordering

Wt. Vol. Conc. Price
50 µg 50 µl - $395

LSBio (Direct) LSBio (Direct)
206-374-1102
866-206-6909
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IGF-II / IGF2 Antibody LS-B7415 Images

[click image for a larger image and more details]
Immunohistochemistry
Anti-IGF2 antibody IHC of human brain, cerebellum, Purkinje.
Western blot
Western Blot (reducing) of IGF-II / IGF2 antibody LS-B7415
Western blot
Western Blot (non-reducing) of IGF-II / IGF2 antibody LS-B7415
Enzyme-Linked Immunosorbent Assay
Sandwich ELISA of IGF-II / IGF2 antibody LS-B7415

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Rabbit Polyclonal (IgG) to Human IGF-II / IGF2
Human
IHC - Paraffin, Western blot, ELISA, Neutralization
Unconjugated

Details

Human IGF-II / IGF2
Rabbit
Human (tested or 100% immunogen sequence identity)
IgG Polyclonal
Immunoaffinity purified

Applications

IHC - Paraffin (5 µg/ml), Western blot (0.1 - 0.2 µg/ml), ELISA (0.5 - 2 µg/ml), Neutralization (3 - 5 µg/ml)

Specificity and Use

IGF-II / IGF2 antibody was raised against e.coli derived recombinant Human IGF-II.
Human IGF-II
Immunohistochemistry: LS-B7415 in an IHC-plus antibody, validated by our pathologists for use in IHC against formalin-fixed, paraffin-embedded human tissues (IHC validation procedure). A concentration of 5 ug/ml is recommended. Neutralization: To yield one-half maximal inhibition [ND] of the biological activity of hIGF-II (10 ng/ml), a concentration of 3.1-4.6 ug/ml of this antibody is required. ELISA: To detect hIGF-II by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.5-2 ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with Biotinylated Anti-Human IGF-II (LS-C104384) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant hIGF-II. Western Blot: To detect hIGF-II by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hIGF-II is 1.5-3 ng/lane, under either reducing or non-reducing conditions.

Packaging

Lyophilized from PBS, pH 7.2
50 µl Sterile water
Store Lyophilized at room temperature up to 1 month; Reconstituted for up to two weeks at 2-8°C. Aliquot and freeze at -20°C for long term storage. Avoid freeze/thaw cycles.
For research use only.

About IGF-II / IGF2

P01344 NM_000612 NP_000603.1

IGF2 Antibody, C11orf43 Antibody, Insulin-like growth factor II Antibody, IGF-II Antibody, PP9974 Antibody, Somatomedin-A Antibody


Immunohistochemistry

Immunohistochemistry
Anti-IGF2 antibody IHC of human brain, cerebellum, Purkinje. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B7415 concentration 5 ug/ml.

Western blot

Western blot
Western Blot (reducing) of IGF-II / IGF2 antibody LS-B7415

Western blot

Western blot
Western Blot (non-reducing) of IGF-II / IGF2 antibody LS-B7415

Enzyme-Linked Immunosorbent Assay

Enzyme-Linked Immunosorbent Assay
Sandwich ELISA of IGF-II / IGF2 antibody LS-B7415

Requested From: United States
Date Requested: 7/3/2015

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