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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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HSF1 Antibody (aa1‑503, Azide‑free) LS‑C7380
HSF1 antibody LS-C7380 is an unconjugated rat monoclonal antibody to HSF1 from human, mouse and rat. Validated for GS, IF, IHC, IP and WB.
Catalog
Size
Price
LS-C7380-100
100 µg
$695
Description
HSF1 antibody LS-C7380 is an unconjugated rat monoclonal antibody to HSF1 from human, mouse and rat. Validated for GS, IF, IHC, IP and WB.
Target
Mouse HSF1
Host
Rat
Reactivity
Mouse, Human, Rat (tested or 100% immunogen sequence identity)
Clonality
IgG1 Monoclonal
Conjugations
Unconjugated. Also available conjugated with AP, APC, Biotin, FITC, HRP, PE.
Purification
Ammonium sulfate precipitation
Modifications
Azide-free
Applications
  • IHC
  • Immunofluorescence
  • Western blot (1 - 2 µg/ml)
  • Immunoprecipitation
  • Gel shift
  • (applications tested for the base form of this product only)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
HSF1 antibody was raised against recombinant protein corresponding to aa1-503 of mouse HSF1. Epitope: aa425-439 of mouse HSF1.
Epitope
aa1-503
Specificity
Recognizes Heat Shock Factor 1 (HSF1) at 70-85kD, depending on the source and state of cells. Species cross-reactivity: Human, mouse and rat.
Usage
Suitable for use in Gel Supershift, Immunofluorescence, Immunoprecipitation, Western Blot. Not suitable for use in Immunohistochemistry. Gel Supershift: Antibody should be used at high concentration (1 mg/ml). Immunoprecipitation: 2 ug antibody/mg protein lysate using Protein G. Western Blot: 1-2 ug/ml. The applications listed have been tested for the unmodified form of this product. Other forms have not been tested.
Presentation
PBS, pH 7.4
Storage
Short term: 4°C; Long term: Add glycerol (40-50%) -20°C.
Restrictions
For research use only.
About HSF1
Q00613 NM_005526 NP_005517.1

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Requested From: United States
Date Requested: 12/18/2018
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