Mouse, Human, Rat
(tested or 100% immunogen sequence identity)
Also available conjugated with AP
Ammonium sulfate precipitation
- Western blot (1 - 2 µg/ml)
- Gel shift
- (applications tested for the base form of this product only)
See our complete line of Immunohistochemistry Reagents
including antigen retrieval solutions, blocking agents
ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
HSF1 antibody was raised against recombinant protein corresponding to aa1-503 of mouse HSF1. Epitope: aa425-439 of mouse HSF1.
Recognizes Heat Shock Factor 1 (HSF1) at 70-85kD, depending on the source and state of cells. Species cross-reactivity: Human, mouse and rat.
Suitable for use in Gel Supershift, Immunofluorescence, Immunoprecipitation, Western Blot. Not suitable for use in Immunohistochemistry. Gel Supershift: Antibody should be used at high concentration (1 mg/ml). Immunoprecipitation: 2 ug antibody/mg protein lysate using Protein G. Western Blot: 1-2 ug/ml. The applications listed have been tested for the unmodified form of this product. Other forms have not been tested.
Short term: 4°C; Long term: Add glycerol (40-50%) -20°C.
For research use only.