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IHCPlus™ HSF1 Antibody Polyclonal IHC,WB LS‑B2682

Catalog Number Size Price
LS-B2682-50 50 µg (1 mg/ml) $485 
Anti-HSF1 antibody IHC of human uterus. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.

IHCPlus™ HSF1 Antibody Polyclonal IHC,WB LS‑B2682

Note: This antibody replaces LS-C7378
Antibody:
Rabbit Polyclonal to Human HSF1
Application:
IHC, IHC-P, WB
Reactivity:
Human, Monkey, Mouse, Rat, Rabbit
Format:
Unconjugated, Unmodified
Price
Catalog Number
$485 
LS-B2682-50
Toll Free North America
866-819-4732

Overview

Antibody:
Rabbit Polyclonal to Human HSF1
Application:
IHC, IHC-P, WB
Reactivity:
Human, Monkey, Mouse, Rat, Rabbit
Format:
Unconjugated, Unmodified

Specifications

Description
HSF1 antibody LS-B2682 is an unconjugated rabbit polyclonal antibody to HSF1 from human, mouse, rat and other species. Validated for IHC and WB. Tested on 20 paraffin-embedded human tissues.
Target
Human HSF1
Host
Rabbit
Reactivity
Human, Monkey, Mouse, Rat, Rabbit (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated
Purification
Protein A purified
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin (5 µg/ml)
  • Western blot (1:500)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
Recombinant human HSF-1 expressed in E. coli
Specificity
Recognizes human HSF-1 at ~85kD protein in unstressed cells and an ~95kD protein in heat shocked cells, corresponding to the molecular masses of the inactive and activated forms respectively of HSF-1. Species cross-reactivity: mouse, rat, monkey, rabbit.
Usage
Immunohistochemistry: LS-B2682 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B2682 was determined to be 5 ug/ml. Western Blot: Positive control: Recombinant Human HSF-1 Protein.
Presentation
PBS, 0.09% Sodium Azide, 50% Glycerol
Storage
Store at 4°C or -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About HSF1
Q00613 NM_005526 NP_005517.1

Validation

Anti-HSF1 antibody IHC of human uterus. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
IHC Validated
Anti-HSF1 antibody IHC of human uterus. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
Anti-HSF1 antibody IHC of human uterus. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.

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Requested From: United States
Date Requested: 9/20/2019