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Anti-HSBP1 Antibody LS-C204524

Ordering

Wt. Vol. Conc. Price
- 100 µl - $285
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)
206-374-1102
866-206-6909
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Most Popular HSBP1 Antibodies

Anti-HSBP1 Antibody (N-Terminus) LS-C101557
Rabbit Polyclonal (IgG) to Human HSBP1
Human, Monkey, Mouse, Rat, Bat, Bovine, Dog, Horse, Pig, Rabbit, Zebrafish
Western blot
Unconjugated
Western blot Image
Anti-HSBP1 Antibody (clone 2C3) LS-C197459
Mouse Monoclonal [clone 2C3] (IgG2a,k) to Human HSBP1
Human
Western blot, ELISA
Unconjugated
Anti-HSBP1 Antibody IHC-plus™ LS-B12278
Rabbit Polyclonal (IgG) to Human HSBP1
Human
IHC - Paraffin, Western blot, ELISA
Unconjugated
Immunohistochemistry - Paraffin Image

100% Guaranteed 100% Guaranteed
Chicken Polyclonal (IgY) to Human HSBP1
Human, Mouse, Rat, Bovine, Pig
ICC, Immunofluorescence, Western blot
Unconjugated

Details

Human HSBP1
Chicken
Human, Mouse, Rat, Bovine, Pig (tested or 100% immunogen sequence identity)
IgY Polyclonal
Unconjugated
Purified
Unmodified

Applications

  • ICC (1:2000)
  • Immunofluorescence (1:2000)
  • Western blot (1:5000)

Specificity and Use

HSBP1 antibody was raised against recombinant full length purified HSP27 from E. coli.
This antibody is known to react with HSP27 from human, cow, pig, mouse, rat and other mammals. Since HSP27 is highly conserved, it is likely that the antibody is effective on other species also.
The antibody solution can be used at dilutions of at least 1:2000 in immunofluorescence experiments. In western blotting using chemiluminescence it can be used at dilutions of 1:5000 or lower.

Packaging

10 mM sodium azide.
+4°C or -20°C, Avoid repeated freezing and thawing.
For research use only.

About HSBP1

O75506 NM_001537 NP_001528.1

HSBP1 Antibody, nasopharyngeal carcinoma-associated antigen 13 Antibody

The heat-shock response is elicited by exposure of cells to thermal and chemical stress and through the activation of HSFs (heat shock factors) results in the elevated expression of heat-shock induced genes. Heat shock factor binding protein 1 (HSBP1), is a 76-amino-acid protein that binds to heat shock factor 1(HSF1), which is a transcription factor involved in the HS response.

Immunocytochemistry

Immunocytochemistry
HeLa cells stained with HSBP1 antibody (green), and counterstained with monoclonal antibody to High mobility Group B protein 1 (HMGB1, red) 1F3 and DNA (blue). HSBP1 antibody antibody reveals strong cytoplasmic staining and penetrates into the actin rich ruffled margins, while the HMGB1 antibody reveals strong nuclear staining which overlaps with the DNA staining.

Western blot

Western blot
Blot of HeLa cell crude extracts blotted with HSBP1 antibody. Note the strong clean bands at 27kDa corresponding to HSP27.

Requested From: United States
Date Requested: 12/10/2016

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