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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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HRH3 / Histamine 3 Receptor Antibody (Cytoplasmic Domain) IHC‑plus™ LS‑A475
Histamine 3 Receptor antibody LS-A475 is an unconjugated rabbit polyclonal antibody to human Histamine 3 Receptor (HRH3). Validated for IHC and WB. Tested on 20 paraffin-embedded human tissues.
Catalog
Size
Price
LS-A475-50
50 µg (1 mg/ml)
$425
In Stock for Immediate Shipment

Popular HRH3 / Histamine 3 Receptor Products

Anti-HRH3 / Histamine H3 Receptor antibody LS-A476 immunocytochemistry (ICC) staining of HEK293 human embryonic kidney cells transfected with HRH3 / Histamine H3 Receptor.
Species: Human, Monkey
Applications: IHC, IHC - Paraffin, ICC
Anti-Histamine 3 Receptor / HRH3 antibody IHC of human brain, neurons. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-A3262 dilution 7-15 ug/ml.
Species: Human, Monkey, Mouse, Rat, Bovine, Guinea pig, Hamster, Horse
Applications: IHC, IHC - Paraffin
Formalin-fixed and paraffin-embedded human brain tissue reacted with HRH3 antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Species: Human
Applications: IHC, IHC - Paraffin, Western blot
Anti-HRH3 / Histamine 3 Receptor antibody IHC staining of human prostate. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B11188 concentration 5 ug/ml.
Species: Human, Bovine, Dog, Guinea pig, Hamster, Horse
Applications: IHC, IHC - Paraffin, Western blot, Peptide Enzyme-Linked Immunosorbent Assay

Product Description

Histamine 3 Receptor antibody LS-A475 is an unconjugated rabbit polyclonal antibody to human Histamine 3 Receptor (HRH3). Validated for IHC and WB. Tested on 20 paraffin-embedded human tissues.

Specifications

Target
Human HRH3 / Histamine 3 Receptor
Synonyms
HRH3, G protein-coupled receptor 97, GPCR97, H3 histamine receptor, H3R, HH3R, Histamine H3 receptor, Histamine receptor H3, G-protein coupled receptor 97, Histamine 3 receptor
Host
Rabbit
Reactivity
Human (tested or 100% immunogen sequence identity)
Predicted
Monkey, Hamster, Horse (at least 90% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated. Also available conjugated with AP, APC, Biotin, FITC, HRP, PE.
Purification
Immunoaffinity purified
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin (2 - 8 µg/ml)
  • Western blot
  • (applications tested for the base form of this product only)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
Synthetic 18 amino acid peptide from 3rd cytoplasmic domain of human HRH3 / Histamine H3 Receptor. Percent identity with other species by BLAST analysis: Human, Gorilla, Gibbon (100%); Monkey, Hamster, Horse (94%); Mouse, Rat (89%); Elephant (83%).
Blocking Peptide
LS-E28986 - Liquid - 50 µg - $145.00
Immunizing peptide used to generate LS-A475. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
Epitope
Cytoplasmic Domain
Specificity
Human HRH3 / Histamine H3 Receptor. BLAST analysis of the peptide immunogen showed no homology with other human proteins.
Presentation
PBS, 0.1% Sodium Azide
Storage
Aliquot and store undiluted at -20°C or below for up to 1 year. Can be stored undiluted at 4°C for up to 1 month. Avoid freeze-thaw cycles.
Restrictions
For research use only.
About HRH3 / Histamine 3 Receptor
The H3 subclass of histamine receptors could mediate the histamine signals in CNS and peripheral nervous system. Signals through the inhibition of adenylate cyclase and displays high constitutive activity (spontaneous activity in the absence of agonist). Agonist stimulation of isoform 3 neither modified adenylate cyclase activity nor induced intracellular calcium mobilization. Q9Y5N1 NM_007232 NP_009163.2


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Images

Immunohistochemistry

Anti-HRH3 / Histamine H3 Receptor antibody LS-A475 IHC of human brain, neurons and glia. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
Anti-HRH3 / Histamine H3 Receptor antibody LS-A475 IHC of human brain, neurons and glia. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.

Immunohistochemistry

Anti-HRH3 / Histamine H3 Receptor antibody LS-A475 IHC of human brain, neurons and glia. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
Anti-HRH3 / Histamine H3 Receptor antibody LS-A475 IHC of human brain, neurons and glia. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.

Immunohistochemistry

Anti-HRH3 / Histamine H3 Receptor antibody LS-A475 IHC of human brain, neurons and glia. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
Anti-HRH3 / Histamine H3 Receptor antibody LS-A475 IHC of human brain, neurons and glia. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.

Immunohistochemistry

Anti-HRH3 / Histamine H3 Receptor antibody LS-A475 IHC of human brain, neurons and glia. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
Anti-HRH3 / Histamine H3 Receptor antibody LS-A475 IHC of human brain, neurons and glia. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.

Requested From: United States
Date Requested: 10/19/2018

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