Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to
worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting
immunostaining in relation to complex human pathologies.
TCR Screening Services
Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding.
Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".
Human, Monkey, Mouse, Rat (tested or 100% immunogen sequence identity)
Hamster, Chicken, Xenopus, Zebrafish (at least 90% immunogen sequence identity)
Western blot (1:1000)
Specificity and Use
HRAS / H-Ras antibody was raised against synthetic peptide corresponding to residues close to the amino-terminus of human K-Ras (KLH coupled).
Detects endogenous levels of total K-Ras, H-Ras and N-Ras. May also cross-react with R-Ras and M-Ras. Species cross-reactivity: Human, mouse, rat. Species sequence Homology: Chicken, hamster, Xenopus, zebra fish: 100%.
Suitable for use in ELISA and Western Blot. Western Blot: 1:1000 (~10blots/100ul).
10 mM sodium HEPES, pH 7.5, 150 mM sodium chloride, 0.1 mg/ml BSA, 50% glycerol.
Long term: -20°C; Short term: +4°C. Avoid repeat freeze-thaw cycles.
HRAS / H-Ras belongs to the Ras oncogene family, whose members are related to the transforming genes of mammalian sarcoma retroviruses. The products encoded by these genes function in signal transduction pathways. These proteins can bind GTP and GDP, and they have intrinsic GTPase activity. This protein undergoes a continuous cycle of de- and re-palmitoylation, which regulates its rapid exchange between the plasma membrane and the Golgi apparatus.