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Catalog Number Size Price
LS-C38766-50 50 µg $604 
Antibody

Monoclonal Rat anti‑Human HRAS / H‑Ras Antibody (aa62‑76, IHC, IF, WB) LS‑C38766

Monoclonal Rat anti‑Human HRAS / H‑Ras Antibody (aa62‑76, IHC, IF, WB) LS‑C38766

Antibody:
HRAS / H-Ras Rat anti-Human Monoclonal (aa62-76) Antibody
Application:
IHC, IHC-P, IHC-Fr, IF, WB, IP, Neut
Reactivity:
Human, Mouse, Rat
Format:
Unconjugated, Unmodified
Price
Catalog Number
$604
LS-C38766-50
Toll Free North America
866-819-4732
For Research Use Only

Overview

Antibody:
HRAS / H-Ras Rat anti-Human Monoclonal (aa62-76) Antibody
Application:
IHC, IHC-P, IHC-Fr, IF, WB, IP, Neut
Reactivity:
Human, Mouse, Rat
Format:
Unconjugated, Unmodified

Specifications

Description
H-Ras antibody LS-C38766 is an unconjugated rat monoclonal antibody to H-Ras (HRAS) (aa62-76) from human. It is reactive with human, mouse and rat. Validated for IF, IHC, IP, Neut and WB.
Target
Human HRAS / H-Ras
Synonyms
HRAS | C-H-RAS | C-has/bas p21 protein | C-ras-Ki-2 activated oncogene | C-HA-RAS1 | CTLO | H-ras | H-RASIDX | GTP- and GDP-binding peptide B | GTPase HRas | H-Ras-1 | HAMSV | HRAS1 | K-RAS | Ha-Ras | Ha-Ras1 proto-oncoprotein | Transforming protein p21 | N-ras | p21ras | RASH1 | C-BAS/HAS | C-ha-ras | p19 H-RasIDX protein
Host
Rat
Reactivity
Human, Mouse, Rat (tested or 100% immunogen sequence identity)
Clonality
IgG1,k Monoclonal
Conjugations
Unconjugated
Purification
Purified
Modifications
Unmodified
Immunogen
Recombinant p21 ras protein. Epitope: aa62-76 of human ras.
Epitope
aa62-76
Specificity
Recognizes phosphorylated and non-phosphorylated human, rat and mouse ras. Does not cross-react with the rap protein. Neutralizes the biological and biochemical activities by binding to residues E63, S65, A66, M67, Q70 and R73. Positive Controls: SW480 or Y1 cells (IF and IP); normal skin (IHC, frozen and paraffin); A431, Hela or ML-1 cells (WB).
Applications
  • IHC
  • IHC - Paraffin
  • IHC - Frozen
  • Immunofluorescence (2.5 µg/ml)
  • Western blot (2.5 µg/ml)
  • Immunoprecipitation
  • Neutralization
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Usage
Suitable for use in Immunofluorescence, Western Blot, Immunoprecipitation, Immunohistochemistry and Neutralization. Immunofluorescence: 2.5 ug/ml. Western Blot (chemiluminescence detection): 2.5 ug/ml. Immunohistochemistry (Frozen): 5 ug/ml. Immunohistochemistry: 1-5 ug/ml. Staining of formalin fixed, paraffin embedded tissue requires pepsin pretreatment. Use saponin to permeablize. Immunoprecipitation (autoradiographic detection): 1 ug/sample SW480 or Y1 cells. Use rabbit anti-rat IgG plus Protein A or goat anti-rat IgG plus Protein G. Protein A alone will not immunoprecipitate rat IgG. Doublets corresponding to farnesylated- and non-farnesylated ras may be observed. Detects both phosphorylated and non-phosphorylated human ras as well as the p21 translational products of the H-, K- and N-ras human genes.
Presentation
PBS, pH 7.4, 0.1% Sodium Azide
Storage
Short term: store at 4°C. Long term: store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About HRAS / H-Ras
P01112 NM_005343 NP_005334.1

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Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 9/16/2021