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Anti-HRAS / H-Ras Antibody (aa104-128) LS-C96691

Ordering

Wt. Vol. Conc. Price
- 400 µl - Unavailable

Most Popular HRAS / H-Ras Antibodies

Anti-HRAS / H-Ras Antibody (aa146-176) LS-C165008
Rabbit Polyclonal to Human HRAS / H-Ras
Human, Mouse
Immunofluorescence, Western blot
Unconjugated
Immunofluorescence Image
Anti-HRAS / H-Ras Antibody (clone 1D9) LS-C174464
Mouse Monoclonal [clone 1D9] (IgG1) to Human HRAS / H-Ras
Human, Monkey, Mouse, Rat, Dog
IHC - Paraffin, Immunofluorescence, Western blot
Unconjugated
Immunohistochemistry Image
Anti-HRAS / H-Ras Antibody (clone H-Ras-03) LS-C188430
Mouse Monoclonal [clone H-Ras-03] (IgG1) to Human HRAS / H-Ras
Human, Monkey, Mouse, Rat, Bovine, Dog, Guinea pig, Hamster, Horse, Pig, Chicken, Xenopus, Fish, Zebrafish
Western blot, ELISA
Unconjugated

100% Guaranteed 100% Guaranteed
Rabbit Polyclonal to Human HRAS / H-Ras
Human
IHC - Paraffin, Immunofluorescence, Western blot
Unconjugated

Details

Human HRAS / H-Ras
Rabbit
Human (tested or 100% immunogen sequence identity)
Mouse, Rat (at least 90% immunogen sequence identity)
Polyclonal
Unconjugated
Ammonium sulfate precipitation
Unmodified

Applications

  • IHC - Paraffin (1:10 - 1:50)
  • Immunofluorescence (1:10 - 1:50)
  • Western blot (1:1000)

Specificity and Use

LS-E9246 - Lyophilized - 100 µg - $145.00
aa104-128
This HRAS antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 104-128 amino acids from the Central region of human HRAS.

Packaging

PBS, 0.09% sodium azide
Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C.
For research use only.

About HRAS / H-Ras

P01112 NM_005343 NP_005334.1

HRAS Antibody, C-H-RAS Antibody, C-has/bas p21 protein Antibody, C-ras-Ki-2 activated oncogene Antibody, C-HA-RAS1 Antibody, CTLO Antibody, H-ras Antibody, H-RASIDX Antibody, GTP- and GDP-binding peptide B Antibody, GTPase HRas Antibody, H-Ras-1 Antibody, HAMSV Antibody, HRAS1 Antibody, K-RAS Antibody, Ha-Ras Antibody, Ha-Ras1 proto-oncoprotein Antibody, Transforming protein p21 Antibody, N-ras Antibody, p21ras Antibody, RASH1 Antibody, C-BAS/HAS Antibody, C-ha-ras Antibody, p19 H-RasIDX protein Antibody

HRAS / H-Ras belongs to the Ras oncogene family, whose members are related to the transforming genes of mammalian sarcoma retroviruses. The products encoded by these genes function in signal transduction pathways. These proteins can bind GTP and GDP, and they have intrinsic GTPase activity. This protein undergoes a continuous cycle of de- and re-palmitoylation, which regulates its rapid exchange between the plasma membrane and the Golgi apparatus.

Immunohistochemistry

Immunohistochemistry
Formalin-fixed and paraffin-embedded human lung carcinoma tissue reacted with HRAS antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

Immunofluorescence

Immunofluorescence
Confocal immunofluorescence of HRAS Antibody with MCF-7 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red). DAPI was used to stain the cell nuclear (blue).

Western blot

Western blot
Western blot of lysate from 293T cell line, using HRAS Antibody. Antibody was diluted at 1:1000. A goat anti-rabbit IgG H&L (HRP) at 1:10000 dilution was used as the secondary antibody. Lysate at 20ug.

Western blot

Western blot
Western blot of anti-HRAS Antibody in Jurkat cell line lysates (35 ug/lane). HRAS (arrow) was detected using the purified antibody.

Requested From: 
Date Requested: 3/23/2017

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