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Anti-HLA-B27 Antibody (clone HLA-ABC-m3, FITC) LS-C57224


Wt. Vol. Conc. Price
- 100 tst - $490
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)

Most Popular HLA-B27 Antibodies

Anti-HLA-B27 Antibody (clone HLA-ABC-m3, FITC) LS-C57224
Mouse Monoclonal [clone HLA-ABC-m3] (IgG2a) to Human HLA-B27
Flow Cytometry
FITC Conjugated
Anti-HLA-B27 Antibody (clone EP-4) LS-C129851
Mouse Monoclonal [clone EP-4] (IgM,k) to Human HLA-B27
ICC, Western blot
Anti-HLA-B27 Antibody (clone EP-4) LS-C204180
Mouse Monoclonal [clone EP-4] (IgM,k) to Human HLA-B27

100% Guaranteed 100% Guaranteed
Mouse Monoclonal [clone HLA-ABC-m3] (IgG2a) to Human HLA-B27
Flow Cytometry
FITC Conjugated


Human HLA-B27
Human (tested or 100% immunogen sequence identity)
IgG2a Monoclonal [HLA-ABC-m3]
Ion exchange chromatography


Flow Cytometry (1:1)

Specificity and Use

HLA-B27 antibody was raised against immune complex precipitated from HLA-B27 positive cell line (Bordin) by anti HLA-antibody and Staphylococcal Protein A.
Recognizes the HLA-B27 alloantigen. Reacts with the peripheral blood lymphocytes of 47/47 individuals conventionally typed as HLA-B27 + and precipitates cell surface molecules of 43 and 12 kD, corresponding to the HLA Class 1 heavy chain and beta 2 microglobulin. Affinity studies by Scatchard analysis showed that this has a higher affinity for HLA-B27 (9.7 x 108 M-1) than for HLA B7 (9.5 x 107 M-1). Subjects Expected Fluorescence Intensity Heterozygous HLA-B27+ Strong Homozygous HLA-B27+ Strong Heterozygous HLA-B7+ Faint Non B27, Non B7 Negative.
Flow Cytometry: Use 10 ul of the suggested working dilution to label 10^6 cells or 100 ul of whole blood. Method sheets are available on request.


PBS, pH 7.4, 0.09% sodium azide, 0.2% BSA.
+4°C, avoid freezing
For research use only.

About HLA-B27

Requested From: United States
Date Requested: 10/25/2016

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