Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to
worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting
immunostaining in relation to complex human pathologies.
TCR Screening Services
Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding.
Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".
Mouse Monoclonal [clone HLA-ABC-m3] (IgG2a) to Human HLA-B27
Human (tested or 100% immunogen sequence identity)
IgG2a Monoclonal [HLA-ABC-m3]
Ion exchange chromatography
Flow Cytometry (1:1)
Specificity and Use
HLA-B27 antibody was raised against immune complex precipitated from HLA-B27 positive cell line (Bordin) by anti HLA-antibody and Staphylococcal Protein A.
Recognizes the HLA-B27 alloantigen. Reacts with the peripheral blood lymphocytes of 47/47 individuals conventionally typed as HLA-B27 + and precipitates cell surface molecules of 43 and 12 kD, corresponding to the HLA Class 1 heavy chain and beta 2 microglobulin. Affinity studies by Scatchard analysis showed that this has a higher affinity for HLA-B27 (9.7 x 108 M-1) than for HLA B7 (9.5 x 107 M-1). Subjects Expected Fluorescence Intensity Heterozygous HLA-B27+ Strong Homozygous HLA-B27+ Strong Heterozygous HLA-B7+ Faint Non B27, Non B7 Negative.
Flow Cytometry: Use 10 ul of the suggested working dilution to label 10^6 cells or 100 ul of whole blood. Method sheets are available on request.