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Anti-HLA-B27 Antibody (clone HLA-ABC-m3, FITC) LS-C57224


Wt. Vol. Conc. Price
- 100 tst - Unavailable

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100% Guaranteed 100% Guaranteed
Mouse Monoclonal [clone HLA-ABC-m3] (IgG2a) to Human HLA-B27
Flow Cytometry
FITC Conjugated


Human HLA-B27
Human (tested or 100% immunogen sequence identity)
IgG2a Monoclonal [HLA-ABC-m3]
Ion exchange chromatography


Flow Cytometry (1:1)

Specificity and Use

HLA-B27 antibody was raised against immune complex precipitated from HLA-B27 positive cell line (Bordin) by anti HLA-antibody and Staphylococcal Protein A.
Recognizes the HLA-B27 alloantigen. Reacts with the peripheral blood lymphocytes of 47/47 individuals conventionally typed as HLA-B27 + and precipitates cell surface molecules of 43 and 12 kD, corresponding to the HLA Class 1 heavy chain and beta 2 microglobulin. Affinity studies by Scatchard analysis showed that this has a higher affinity for HLA-B27 (9.7 x 108 M-1) than for HLA B7 (9.5 x 107 M-1). Subjects Expected Fluorescence Intensity Heterozygous HLA-B27+ Strong Homozygous HLA-B27+ Strong Heterozygous HLA-B7+ Faint Non B27, Non B7 Negative.
Flow Cytometry: Use 10 ul of the suggested working dilution to label 10^6 cells or 100 ul of whole blood. Method sheets are available on request. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.


PBS, pH 7.4, 0.09% sodium azide, 0.2% BSA.
+4°C, avoid freezing
For research use only.

About HLA-B27

Requested From: 
Date Requested: 4/25/2017

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