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Anti-HLA-B27 Antibody (clone HLA-ABC-m3, FITC) LS-C57224

Ordering

Wt. Vol. Conc. Price
- 100 tst - $490
Inquire for larger quantities

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206-374-1102
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Most Popular HLA-B27 Antibodies

Anti-HLA-B27 Antibody (clone HLA-ABC-m3, FITC) LS-C57224
Mouse Monoclonal [clone HLA-ABC-m3] (IgG2a) to Human HLA-B27
Human
Flow Cytometry
FITC Conjugated
Anti-HLA-B27 Antibody (clone EP-4) LS-C129851
Mouse Monoclonal [clone EP-4] (IgM,k) to Human HLA-B27
Human
ICC, Western blot
Unconjugated
Anti-HLA-B27 Antibody (clone EP-4) LS-C204180
Mouse Monoclonal [clone EP-4] (IgM,k) to Human HLA-B27
Human
ICC
Unconjugated

100% Guaranteed 100% Guaranteed
Mouse Monoclonal [clone HLA-ABC-m3] (IgG2a) to Human HLA-B27
Human
Flow Cytometry
FITC Conjugated

Details

Human HLA-B27
Mouse
Human (tested or 100% immunogen sequence identity)
IgG2a Monoclonal [HLA-ABC-m3]
Fluorescein
Ion exchange chromatography
Unmodified

Applications

Flow Cytometry (1:1)

Specificity and Use

HLA-B27 antibody was raised against immune complex precipitated from HLA-B27 positive cell line (Bordin) by anti HLA-antibody and Staphylococcal Protein A.
Recognizes the HLA-B27 alloantigen. Reacts with the peripheral blood lymphocytes of 47/47 individuals conventionally typed as HLA-B27 + and precipitates cell surface molecules of 43 and 12 kD, corresponding to the HLA Class 1 heavy chain and beta 2 microglobulin. Affinity studies by Scatchard analysis showed that this has a higher affinity for HLA-B27 (9.7 x 108 M-1) than for HLA B7 (9.5 x 107 M-1). Subjects Expected Fluorescence Intensity Heterozygous HLA-B27+ Strong Homozygous HLA-B27+ Strong Heterozygous HLA-B7+ Faint Non B27, Non B7 Negative.
Flow Cytometry: Use 10 ul of the suggested working dilution to label 10^6 cells or 100 ul of whole blood. Method sheets are available on request. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.

Packaging

PBS, pH 7.4, 0.09% sodium azide, 0.2% BSA.
+4°C, avoid freezing
For research use only.

About HLA-B27


Requested From: United States
Date Requested: 12/4/2016

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