Human HLA-B27

Mouse

Human (tested or 100% immunogen sequence identity)

IgG2a Monoclonal

HLA-ABC-m3

FITC

Ion exchange chromatography

Unmodified

Immune complex precipitated from HLA-B27 positive cell line (Bordin) by anti HLA-antibody and Staphylococcal Protein A.

Recognizes the HLA-B27 alloantigen. Reacts with the peripheral blood lymphocytes of 47/47 individuals conventionally typed as HLA-B27 + and precipitates cell surface molecules of 43 and 12 kD, corresponding to the HLA Class 1 heavy chain and beta 2 microglobulin. Affinity studies by Scatchard analysis showed that this has a higher affinity for HLA-B27 (9.7 x 108 M-1) than for HLA B7 (9.5 x 107 M-1). Subjects Expected Fluorescence Intensity Heterozygous HLA-B27+ Strong Homozygous HLA-B27+ Strong Heterozygous HLA-B7+ Faint Non B27, Non B7 Negative.

• Flow Cytometry (1:1)

Flow Cytometry: Use 10 ul of the suggested working dilution to label 10^6 cells or 100 ul of whole blood. Method sheets are available on request. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.

PBS, 0.1% Sodium Azide, 0.2% BSA

Store at 4°C. Do not freeze. Store undiluted. Protect from light. Microcentrifugation recommended if solution contains precipitate.

For research use only. Intended for use by laboratory professionals.

This antibody carries the LSBio 100% Guarantee.