(tested or 100% immunogen sequence identity)
Also available conjugated with AP
- Western blot
- Chromatin Immunoprecipitation
- (applications tested for the base form of this product only)
Histone H4 antibody was raised against bSA-conjugated, synthetic peptide containing the sequence. HR[me3K]VL. in which me3K corresponds to trimethyl-lysine 20 of human histone H4.
Recognizes trimethyl-Histone H4 (Lys20), Mr 10kD. Species cross-reactivity: Human and mouse. Broad species cross-reactivity is expected.
Suitable for use in Western Blot and Chromatin IP. Western Blot Analysis: 1:500-1:2000 dilutions of this lot detected methylated histone H4 in acid extracted proteins from HeLa cells, but not demethylated recombinant Histone H4. Chromatin Immunoprecipitation: Has been reported by an independent laboratory to immunoprecipitate chromatin containing trimethylhistone H4 (Lys20).
0.1 M Tris-Glycine, pH 7.4, 0.15 M NaCl, 0.05% Sodium Azide
Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.
For research use only.