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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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Histone H4 Antibody (Tri‑Methyl‑Lys20) LS‑C24402
Histone H4 antibody LS-C24402 is an unconjugated rabbit polyclonal antibody to Histone H4 from human and mouse. Validated for ChrIP and WB.
Antibody
Catalog
Size
Price
LS-C24402-200
200 µl (0.5 mg/ml)
$710

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Species: Human
Applications: IHC, Western blot, ELISA

Product Description

Histone H4 antibody LS-C24402 is an unconjugated rabbit polyclonal antibody to Histone H4 from human and mouse. Validated for ChrIP and WB.
About Histone H4
Histone H4 is one of the 5 main histone proteins involved in the structure of chromatin in eukaryotic cells. Featuring a main globular domain and a long N terminal tail, H4 is a structural component of the nucleosome, and is subject to covalent modification, including acetylation and methylation, which may alter expression of genes located on DNA associated with its parent histone octamer.

Specifications

Target
Human Histone H4
Host
Rabbit
Reactivity
Human, Mouse (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated. Also available conjugated with AP, APC, Biotin, FITC, HRP, PE.
Purification
Antiserum
Modifications
Unmodified
Applications
  • Western blot
  • Chromatin Immunoprecipitation
  • (applications tested for the base form of this product only)
Immunogen
Histone H4 antibody was raised against bSA-conjugated, synthetic peptide containing the sequence. HR[me3K]VL. in which me3K corresponds to trimethyl-lysine 20 of human histone H4.
Epitope
Tri-Methyl-Lys20
Specificity
Recognizes trimethyl-Histone H4 (Lys20), Mr 10kD. Species cross-reactivity: Human and mouse. Broad species cross-reactivity is expected.
Usage
Suitable for use in Western Blot and Chromatin IP. Western Blot Analysis: 1:500-1:2000 dilutions of this lot detected methylated histone H4 in acid extracted proteins from HeLa cells, but not demethylated recombinant Histone H4. Chromatin Immunoprecipitation: Has been reported by an independent laboratory to immunoprecipitate chromatin containing trimethylhistone H4 (Lys20).
Presentation
0.1 M Tris-Glycine, pH 7.4, 0.15 M NaCl, 0.05% Sodium Azide
Storage
Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only.

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Requested From: United States
Date Requested: 9/19/2018

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