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Catalog Number Size Price
LS-B5032-50 50 µg (1 mg/ml) $855 
Histone H4 Antibody - Anti-Histone H4 antibody IHC of human skin. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.

IHC‑plus™ Monoclonal Mouse anti‑Human Histone H4 Antibody (Di‑Methyl‑Lys20, IHC, WB) LS‑B5032

IHC‑plus™ Monoclonal Mouse anti‑Human Histone H4 Antibody (Di‑Methyl‑Lys20, IHC, WB) LS‑B5032

Note: This antibody replaces LS-C24397
Antibody:
Histone H4 Mouse anti-Human Monoclonal (Di-Methyl-Lys20) Antibody
Application:
IHC, IHC-P, WB, IP
Reactivity:
Human, Mouse, Rat, Bovine
Format:
Unconjugated, Unmodified
Other formats:
Price
Catalog Number
$855
LS-B5032-50
Toll Free North America
866-819-4732
For Research Use Only

Overview

Antibody:
Histone H4 Mouse anti-Human Monoclonal (Di-Methyl-Lys20) Antibody
Application:
IHC, IHC-P, WB, IP
Reactivity:
Human, Mouse, Rat, Bovine
Format:
Unconjugated, Unmodified
Other formats:

Specifications

Description
Histone H4 antibody LS-B5032 is an unconjugated mouse monoclonal antibody to Histone H4 (Di-Methyl-Lys20) from human. It is reactive with human, mouse, rat and other species. Validated for IHC, IP and WB. Tested on 20 paraffin-embedded human tissues.
Target
Human Histone H4
Host
Mouse
Reactivity
Human, Mouse, Rat, Bovine (tested or 100% immunogen sequence identity)
Clonality
IgG1 Monoclonal
Conjugations
Unconjugated. Also available conjugated with AP, APC, Biotin, FITC, HRP, PE.
Purification
Protein G purified
Modifications
Unmodified
Immunogen
KLH-conjugated, synthetic peptide corresponding to amino acids 17-28 (RHR[dimeK] VLRDNIQGC) of human histone H4.
Epitope
Di-Methyl-Lys20
Specificity
Recognizes methyl-Histone H4, Mr 10kD. Species cross-reactivity: Human, mouse, rat, and bovine. Broad species Cross-reactivity predicted based on conservation of sequence. Does not cross-react with yeast or Tetrahymena.
Applications
  • IHC
  • IHC - Paraffin (5 µg/ml)
  • Western blot (0.01 - 0.1 µg/ml)
  • Immunoprecipitation
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Usage
Suitable for use in Western Blot, Immunoprecipitation and Immunohistochemistry. Western Blot: 0.01-0.1 ug/ml detects methyl-histone H4 in HeLa acid extracts. Minimal cross-reactivity with unmethylated recombinant Histone H4 is observed if used at these concentrations. Immunohistochemistry: stains Drosophila polytene chromosomes as reported by an independent laboratory. Immunoprecipitation: Immunoprecipitates methyl-Histone H4 from HeLa cell lysates.
Presentation
PBS
Storage
May be stored at 4°C for short-term only. Aliquot to avoid freeze-thaw cycles. Store at -20°C. Aliquots are stable for up to 1 year.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee

Validation

Histone H4 Antibody - Anti-Histone H4 antibody IHC of human skin. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
Anti-Histone H4 antibody IHC of human skin. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
Anti-Histone H4 antibody IHC of human skin. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.

LSBio Ratings

IHC-plus™ Histone H4 Antibody (Di-Methyl-Lys20) for IHC, WB/Western, IP LS-B5032 has an LSBio Rating of
Laboratory Validation Score (4)

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Customer Reviews (0)


Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 1/19/2022