(tested or 100% immunogen sequence identity)
- Western blot (1:1000)
Histone H4 antibody was raised against kLH-conjugated, synthetic peptide (AKRHR(dimeth-K)VLRDN-C), corresponding to amino acids 15-25 of human, mouse and rat Histone H4 dimethylated on lysine 20.
Recognizes dimethyl-Histone H4 (Lys20), Mr ~10kD. Species cross-reactivity: Broad, based on sequence homology.
Suitable for use in Western Blot, Chromatin Immunoprecipitation. Western Blot: 1:1000 to 1:10000 detected dimethyl-Histone H4 (Lys20) in acid extracts from HeLa cells, but not the unmethylated recombinant Histone H4. Chromatin Immunoprecipitation: immunoprecipitates chromatin containing dimethyl-. Histone H4 (Lys20).
Serum, 0.05% Sodium Azide
Short term: 4°C; Long term: Add glycerol (40-50%) -20°C.
For research use only.