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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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Histone H4 Antibody (Acetyl‑Lys5/Lys10/Lys14/Lys18) LS‑C24395
Histone H4 antibody LS-C24395 is an unconjugated rabbit polyclonal antibody to Histone H4 from human and tetrahymena. Validated for ICC, IP and WB.
Catalog
Size
Price
LS-C24395-200
200 µl
$785
Description
Histone H4 antibody LS-C24395 is an unconjugated rabbit polyclonal antibody to Histone H4 from human and tetrahymena. Validated for ICC, IP and WB.
Target
Tetrahymena Histone H4
Host
Rabbit
Reactivity
Tetrahymena, Human (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated
Purification
Antiserum
Modifications
Unmodified
Applications
  • ICC
  • Western blot (1:2000)
  • Immunoprecipitation
Immunogen
Histone H4 antibody was raised against kLH-conjugated peptide [AGGAcKGGAcKGMGAcKVGAAcKRHS-C] corresponding to amino acids 2-19 of Tetrahymena histone H4.
Epitope
Acetyl-Lys5/Lys10/Lys14/Lys18
Specificity
Recognizes acetylated histone H4 of approximately 10kD. Cross-reacts with acetylated histone H2B from Tetrahymena and weakly cross-reacts with acetylated histone H2B from HeLa cells. May cross-react with other acetylated proteins. Cross-reactivity: Human and Tetrahymena. Other species not tested, but expected to cross-react since Histone H4 is well conserved.
Usage
Western Blot: 1:2000 dilution detects acetylated histone H4 in Tetrahymena macronuclei and acid extracted proteins from HeLa cells treated with 5 mM sodium butyrate. Sodium butyrate, an inhibitor of deacetylases, was used to enhance detection of acetylated histone H4. Chromatin Immunoprecipitation: 5-10 ul immunoprecipitates transcriptionally active. chromatin containing acetylated histone H4 from 2X 106 serum stimulated HeLa cells. Immunocytochemistry: Not recommended.
Presentation
Neat Serum, 0.05% Sodium Azide
Storage
Short term: 4°C; Long term: Add glycerol (40-50%) -20°C.
Restrictions
For research use only.

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Requested From: United States
Date Requested: 12/16/2018
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