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| Catalog Number | Size | Price |
|---|---|---|
| LS-C24395-200 | 200 µl | $1,058 |
Polyclonal Rabbit anti‑Tetrahymena Histone H4 Antibody (Acetyl‑Lys5/Lys10/Lys14/Lys18, WB) LS‑C24395
Polyclonal Rabbit anti‑Tetrahymena Histone H4 Antibody (Acetyl‑Lys5/Lys10/Lys14/Lys18, WB) LS‑C24395
Antibody:
Histone H4 Rabbit anti-Tetrahymena Polyclonal (Acetyl-Lys5/Lys10/Lys14/Lys18) Antibody
Application:
ICC, WB, IP
Reactivity:
Tetrahymena, Human
Format:
Unconjugated, Unmodified
Toll Free North America
206-374-1102
206-374-1102For Research Use Only
Overview
Antibody:
Histone H4 Rabbit anti-Tetrahymena Polyclonal (Acetyl-Lys5/Lys10/Lys14/Lys18) Antibody
Application:
ICC, WB, IP
Reactivity:
Tetrahymena, Human
Format:
Unconjugated, Unmodified
Specifications
Description
Histone H4 antibody LS-C24395 is an unconjugated rabbit polyclonal antibody to Histone H4 (Acetyl-Lys5/Lys10/Lys14/Lys18) from tetrahymena. It is reactive with human and tetrahymena. Validated for ICC, IP and WB.
Host
Rabbit
Reactivity
Tetrahymena, Human
(tested or 100% immunogen sequence identity)
Clonality
IgG
Polyclonal
Conjugations
Unconjugated
Purification
Antiserum
Modifications
Unmodified
Immunogen
KLH-conjugated peptide [AGGAcKGGAcKGMGAcKVGAAcKRHS-C] corresponding to amino acids 2-19 of Tetrahymena histone H4.
Epitope
Acetyl-Lys5/Lys10/Lys14/Lys18
Specificity
Recognizes acetylated histone H4 of approximately 10kD. Cross-reacts with acetylated histone H2B from Tetrahymena and weakly cross-reacts with acetylated histone H2B from HeLa cells. May cross-react with other acetylated proteins. Cross-reactivity: Human and Tetrahymena. Other species not tested, but expected to cross-react since Histone H4 is well conserved.
Applications
- ICC
- Western blot (1:2000)
- Immunoprecipitation
Usage
Western Blot: 1:2000 dilution detects acetylated histone H4 in Tetrahymena macronuclei and acid extracted proteins from HeLa cells treated with 5 mM sodium butyrate. Sodium butyrate, an inhibitor of deacetylases, was used to enhance detection of acetylated histone H4. Chromatin Immunoprecipitation: 5-10 ul immunoprecipitates transcriptionally active. chromatin containing acetylated histone H4 from 2X 106 serum stimulated HeLa cells. Immunocytochemistry: Not recommended.
Presentation
Neat Serum, 0.05% Sodium Azide
Storage
Short term: 4°C; Long term: Add glycerol (40-50%) -20°C.
Restrictions
For research use only. Intended for use by laboratory professionals.
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Customer Reviews (0)
Featured Products
Species:
Human, Mouse, Rat, Bovine
Applications:
IHC, IHC - Paraffin, Western blot, Immunoprecipitation
Species:
Human, Mouse, Rat
Applications:
IHC, IHC - Paraffin, Immunofluorescence, Western blot, Peptide Enzyme-Linked Immunosorbent Assay
Species:
Human
Applications:
IHC, Flow Cytometry, ELISA, Dot Blot
Species:
Human
Applications:
IHC, Western blot, Immunoprecipitation, ELISA
Species:
Human, Monkey, Mouse, Rat
Applications:
IHC, IHC - Paraffin, Western blot
Species:
Human
Applications:
IHC, Western blot, ELISA
Request SDS/MSDS
To request an SDS/MSDS form for this product, please contact our Technical Support department at:
Technical.Support@LSBio.com
Requested From: United States
Date Requested: 4/26/2024
Date Requested: 4/26/2024
