Tetrahymena Histone H4
(tested or 100% immunogen sequence identity)
- Western blot (1:2000)
Histone H4 antibody was raised against kLH-conjugated peptide [AGGAcKGGAcKGMGAcKVGAAcKRHS-C] corresponding to amino acids 2-19 of Tetrahymena histone H4.
Recognizes acetylated histone H4 of approximately 10kD. Cross-reacts with acetylated histone H2B from Tetrahymena and weakly cross-reacts with acetylated histone H2B from HeLa cells. May cross-react with other acetylated proteins. Cross-reactivity: Human and Tetrahymena. Other species not tested, but expected to cross-react since Histone H4 is well conserved.
Western Blot: 1:2000 dilution detects acetylated histone H4 in Tetrahymena macronuclei and acid extracted proteins from HeLa cells treated with 5 mM sodium butyrate. Sodium butyrate, an inhibitor of deacetylases, was used to enhance detection of acetylated histone H4. Chromatin Immunoprecipitation: 5-10 ul immunoprecipitates transcriptionally active. chromatin containing acetylated histone H4 from 2X 106 serum stimulated HeLa cells. Immunocytochemistry: Not recommended.
Neat Serum, 0.05% Sodium Azide
Short term: 4°C; Long term: Add glycerol (40-50%) -20°C.
For research use only.