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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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Histone H4 Antibody (Acetyl‑Lys4/Lys7/Lys111/Lys15) LS‑C24380
Histone H4 antibody LS-C24380 is an unconjugated rabbit polyclonal antibody to human Histone H4. Validated for ICC, IP and WB.
Antibody
Catalog
Size
Price
LS-C24380-200
200 µg
$770

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Product Description

Histone H4 antibody LS-C24380 is an unconjugated rabbit polyclonal antibody to human Histone H4. Validated for ICC, IP and WB.
About Histone H4
Histone H4 is one of the 5 main histone proteins involved in the structure of chromatin in eukaryotic cells. Featuring a main globular domain and a long N terminal tail, H4 is a structural component of the nucleosome, and is subject to covalent modification, including acetylation and methylation, which may alter expression of genes located on DNA associated with its parent histone octamer.

Specifications

Target
Histone H4
Host
Rabbit
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated. Also available conjugated with AP, APC, Biotin, FITC, HRP, PE.
Purification
Protein A purified
Modifications
Unmodified
Applications
  • ICC (5 - 10 µg/ml)
  • Western blot (0.5 - 2 µg/ml)
  • Immunoprecipitation
  • (applications tested for the base form of this product only)
Immunogen
Histone H4 antibody was raised against kLH-conjugated peptide AGG[Ac]KGG[AcK]GMG[AcK]VGA[AcK]RHS-C corresponding to the amino acids 2-19 of Tetrahymena histone H4.
Epitope
Acetyl-Lys4/Lys7/Lys111/Lys15
Specificity
Recognizes acetylated histone H4 of approximately 10kD of human and tetrahymena. Cross-reacts with acetylated histone H2B from Tetrahymena and weakly cross-reacts with acetylated histone H2B from HeLa cells. May cross-react with other acetylated proteins.
Usage
Suitable for use in Western Blot, Immunoprecipitation, Immunocytochemistry. Western Blot: 0.5-2 ug/ml will detect acetylated histone H4 in acid-extracted proteins from HeLa cells treated with 5 mM sodium butyrate; a previous lot detected acetylated histone H4 in Tetrahymena macronuclei. Sodium butyrate, an inhibitor of deacetylases, was used to enhance detection of acetylated histone H4. Immunoprecipitation: 5 ug will immunoprecipitate acetylated histone H4 from 500 ug of acid extracted proteins from HeLa cells treated with 5 mM sodium butyrate for 24 hours. Immunocytochemistry: 5-10 ug/ml gave positive nuclear immunostaining in human A431 cells treated with sodium butyrate and fixed with ice-cold 1% paraformaldehyde in PBS. Western Blot Analysis. Acid-extracted proteins from normal HeLa cells and HeLa cells treated with 5 mM sodium butyrate for 24 hours were resolved by electrophoresis, transferred to nitrocellulose and probed with anti-acetylated Histone H4 (1 ug/ml). Proteins were visualized using a goat-anti rabbit secondary antibody conjugated to HRP and a chemiluminescence detection system.
Presentation
0.1 M Tris-Glycine, pH 7.4, 0.15 M NaCl, 0.05% Sodium Azide
Restrictions
For research use only.

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Requested From: United States
Date Requested: 9/19/2018

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