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Anti-Histone H4 Antibody (Acetyl-Lys4/Lys7/Lys111/Lys15) LS-C24380


Wt. Vol. Conc. Price
200 µg - - $650
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)

Most Popular Histone H4 Antibodies

Anti-Histone H4 Antibody (Di-Methyl-Lys20) IHC-plus™ LS-B5032
Mouse Monoclonal (IgG1) to Human Histone H4
Human, Mouse, Rat, Bovine
IHC - Paraffin, Western blot, Immunoprecipitation
Immunohistochemistry Image
Anti-Histone H4 Antibody (Acetyl-Lys5) IHC-plus™ LS-B6121
Rabbit Polyclonal (IgG) to Human Histone H4
Human, Mouse, Rat
IHC - Paraffin, Immunofluorescence, Western blot, ELISA
Immunohistochemistry Image

100% Guaranteed 100% Guaranteed
Rabbit Polyclonal (IgG)
ICC, Western blot, Immunoprecipitation


Histone H4
Human (tested or 100% immunogen sequence identity)
IgG Polyclonal
Protein A purified


  • ICC (5 - 10 µg/ml)
  • Western blot (0.5 - 2 µg/ml)
  • Immunoprecipitation

Specificity and Use

Histone H4 antibody was raised against kLH-conjugated peptide AGG[Ac]KGG[AcK]GMG[AcK]VGA[AcK]RHS-C corresponding to the amino acids 2-19 of Tetrahymena histone H4.
Recognizes acetylated histone H4 of approximately 10kD of human and tetrahymena. Cross-reacts with acetylated histone H2B from Tetrahymena and weakly cross-reacts with acetylated histone H2B from HeLa cells. May cross-react with other acetylated proteins.
Suitable for use in Western Blot, Immunoprecipitation, Immunocytochemistry. Western Blot: 0.5-2 ug/ml will detect acetylated histone H4 in acid-extracted proteins from HeLa cells treated with 5 mM sodium butyrate; a previous lot detected acetylated histone H4 in Tetrahymena macronuclei. Sodium butyrate, an inhibitor of deacetylases, was used to enhance detection of acetylated histone H4. Immunoprecipitation: 5 ug will immunoprecipitate acetylated histone H4 from 500 ug of acid extracted proteins from HeLa cells treated with 5 mM sodium butyrate for 24 hours. Immunocytochemistry: 5-10 ug/ml gave positive nuclear immunostaining in human A431 cells treated with sodium butyrate and fixed with ice-cold 1% paraformaldehyde in PBS. Western Blot Analysis. Acid-extracted proteins from normal HeLa cells and HeLa cells treated with 5 mM sodium butyrate for 24 hours were resolved by electrophoresis, transferred to nitrocellulose and probed with anti-acetylated Histone H4 (1 ug/ml). Proteins were visualized using a goat-anti rabbit secondary antibody conjugated to HRP and a chemiluminescence detection system.


0.1 M Tris-glycine, pH 7.4, 0.15 M sodium chloride, 0.05% sodium azide.
For research use only.

About Histone H4

Histone H4 is one of the 5 main histone proteins involved in the structure of chromatin in eukaryotic cells. Featuring a main globular domain and a long N terminal tail, H4 is a structural component of the nucleosome, and is subject to covalent modification, including acetylation and methylation, which may alter expression of genes located on DNA associated with its parent histone octamer.

Requested From: United States
Date Requested: 10/27/2016

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