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Anti-Histone H4 Antibody (Acetyl) LS-C150104

Ordering

Wt. Vol. Conc. Price
100 µg - - $395
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)
206-374-1102
866-206-6909
Orders@LSBio.com
 

Most Popular Histone H4 Antibodies

Anti-Histone H4 Antibody (Di-Methyl-Lys20) IHC-plus™ LS-B5032
Mouse Monoclonal (IgG1) to Human Histone H4
Human, Mouse, Rat, Bovine
IHC - Paraffin, Western blot, Immunoprecipitation
Unconjugated
Immunohistochemistry Image
Anti-Histone H4 Antibody (Acetyl-Lys5) IHC-plus™ LS-B6121
Rabbit Polyclonal (IgG) to Human Histone H4
Human, Mouse, Rat
IHC - Paraffin, Immunofluorescence, Western blot, ELISA
Unconjugated
Immunohistochemistry Image

100% Guaranteed 100% Guaranteed
Sheep Polyclonal (IgG) to Human Histone H4
Human, Mouse
ICC, Western blot
Unconjugated

Details

Human Histone H4
Sheep
Human, Mouse (tested or 100% immunogen sequence identity)
IgG Polyclonal
Unconjugated
Immunoaffinity purified
Unmodified

Applications

  • ICC (5 - 15 µg/ml)
  • Western blot (1 µg/ml)

Specificity and Use

Histone H4 antibody was raised against tetrahymena pyriformis acetylated Histone H4 synthetic peptide AGGKGGKGMGKVGAKRHSK, Accession # P02310.
Acetyl
Detects immunogen in direct ELISAs and human Acetyl Histone H4 in Western blots. Reactivity with nonacetylated peptide was removed.

Packaging

Lyophilized from 0.2 um filtered solution in PBS, trehalose
Sterile PBS. Resuspend to 500 ul
Long term: -20°C; Short term: +4°C; Avoid freeze-thaw cycles.
For research use only.

About Histone H4

Histone H4 is one of the 5 main histone proteins involved in the structure of chromatin in eukaryotic cells. Featuring a main globular domain and a long N terminal tail, H4 is a structural component of the nucleosome, and is subject to covalent modification, including acetylation and methylation, which may alter expression of genes located on DNA associated with its parent histone octamer.

Immunocytochemistry

Immunocytochemistry
Histone H4 in NTera-2 Human Cell Line. Histone H4 was detected in immersion fixed NTera-2 human testicular embryonic carcinoma cell line using Sheep Anti-Human Acetyl Histone H4 Antigen Affinity-purified Polyclonal Antibody at 10 ug/ml for 3 hours at room temperature. Cells were stained using the NorthernLights 557-conjugated Anti-Sheep IgG Secondary Antibody (red, upper panel) and counterstained with DAPI (blue, lower panel). Specific staining was localized to nuclei.

Western blot

Western blot
Detection of Human Acetyl Histone H4 by Western Blot. Western blot shows lysates of Tera-2 human embryonic lung carcinoma cell line, Nalm-6 human Pre-B acute lymphocytic leukemia cell line, and Jurkat human acute T cell leukemia cell line. PVDF Membrane was probed with 1 ug/ml of Sheep Anti-Human.

Requested From: United States
Date Requested: 12/8/2016

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