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Anti-HIP1 Antibody (clone 1F12) IHC-plus™ LS-B6765

Note: This antibody replaces LS-C133240

Ordering

Wt. Vol. Conc. Price
50 µg - 0.4 mg/ml $450
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)
206-374-1102
866-206-6909
Orders@LSBio.com
 

Most Popular HIP1 Antibodies

Anti-HIP1 Antibody (clone 1F12) IHC-plus™ LS-B6765
Mouse Monoclonal [clone 1F12] (IgG2a,k) to Human HIP1
Human
IHC - Paraffin, Western blot, Immunoprecipitation, ELISA
Unconjugated
Immunohistochemistry Image

100% Guaranteed 100% Guaranteed
Mouse Monoclonal [clone 1F12] (IgG2a,k) to Human HIP1
Human
IHC - Paraffin, Western blot, Immunoprecipitation, ELISA
Unconjugated

Details

Human HIP1
Mouse
Human (tested or 100% immunogen sequence identity)
IgG2a,k Monoclonal [1F12]
Unconjugated
Protein A purified
Unmodified

Applications

  • IHC - Paraffin (10 µg/ml)
  • Western blot
  • Immunoprecipitation
  • ELISA

Specificity and Use

HIP1 antibody was raised against hIP1 (NP_005329, 928 a.a. ~ 1038 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Human HIP1
Immunohistochemistry: Formalin-fixed paraffin-embedded sections. Sandwich ELISA: Recombinant protein. Western Blot: Cell lysate, Transfected lysate, Recombinant protein.

Packaging

PBS, pH 7.2. Sourced in Ascites.
Short term 4°C, long term aliquot and store at -20°C, avoid freeze thaw cycles.
For research use only.

About HIP1

O00291 NM_005338 NP_005329.3

HIP1 Antibody, HIP-1 Antibody, HIP-I Antibody, ILWEQ Antibody

Plays a role in clathrin-mediated endocytosis and trafficking. Involved in regulating AMPA receptor trafficking in the central nervous system in an NMDA-dependent manner. Enhances androgen receptor (AR)-mediated transcription. May act as a proapoptotic protein that induces cell death by acting through the intrinsic apoptosis pathway. Binds 3-phosphoinositides (via ENTH domain).

Immunohistochemistry

Immunohistochemistry
Anti-HIP1 antibody IHC of human kidney, tubules. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B6765 concentration 10 ug/ml.

Western blot

Western blot
HIP1 monoclonal antibody, clone 1F12 Western blot of HIP1 expression in HeLa.

Western blot

Western blot
Western blot of HIP1 expression in transfected 293T cell line by HIP1 monoclonal antibody, clone 1F12. Lane 1: HIP1 transfected lysate (116.2 kD). Lane 2: Non-transfected lysate.

Immunoprecipitation

Immunoprecipitation
Immunoprecipitation of HIP1 transfected lysate using anti-HIP1 monoclonal antibody and Protein A Magnetic Bead immunoblotted with HIP1 rabbit polyclonal antibody.

Enzyme-Linked Immunosorbent Assay

Enzyme-Linked Immunosorbent Assay
Detection limit for recombinant GST tagged HIP1 is approximately 0.3 ng/ml as a capture antibody.
 

Requested From: United States
Date Requested: 12/6/2016

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